2NLA

Crystal structure of the Mcl-1:mNoxaB BH3 complex

2NLA の概要

• エントリーDOI: 10.2210/pdb2nla/pdb
• 関連するPDBエントリー: 1WSX 2NL9
• 分子名称: FUSION PROTEIN CONSISTING OF Induced myeloid leukemia cell differentiation protein Mcl-1 homolog, Phorbol-12-myristate-13-acetate-induced protein 1 (3 entities in total)
• 機能のキーワード: apoptosis, bcl-2, mcl-1, noxa
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Membrane ; Single-pass membrane protein : Q07820; Mitochondrion : Q9JM54
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20829.65

構造登録者

Czabotar, P.E.,Colman, P.M. (登録日: 2006-10-19, 公開日: 2007-03-27, 最終更新日: 2024-11-20)

主引用文献

Czabotar, P.E.,Lee, E.F.,van Delft, M.F.,Day, C.L.,Smith, B.J.,Huang, D.C.,Fairlie, W.D.,Hinds, M.G.,Colman, P.M.
Structural insights into the degradation of Mcl-1 induced by BH3 domains.
Proc.Natl.Acad.Sci.USA, 104:6217-6222, 2007

PubMed Abstract: Apoptosis is held in check by prosurvival proteins of the Bcl-2 family. The distantly related BH3-only proteins bind to and antagonize them, thereby promoting apoptosis. Whereas binding of the BH3-only protein Noxa to prosurvival Mcl-1 induces Mcl-1 degradation by the proteasome, binding of another BH3-only ligand, Bim, elevates Mcl-1 protein levels. We compared the three-dimensional structures of the complexes formed between BH3 peptides of both Bim and Noxa, and we show that a discrete C-terminal sequence of the Noxa BH3 is necessary to instigate Mcl-1 degradation.

PubMed: 17389404
DOI: 10.1073/pnas.0701297104

実験手法

X-RAY DIFFRACTION (2.8 Å)