2NAX

Structure of CCHC zinc finger domain of Pcf11

2NAX の概要

• エントリーDOI: 10.2210/pdb2nax/pdb
• NMR情報: BMRB: 25953
• 分子名称: Protein PCF11, ZINC ION (2 entities in total)
• 機能のキーワード: zinc finger, metal binding protein
• 由来する生物種: Saccharomyces cerevisiae S288c (Baker's yeast)
• 細胞内の位置: Nucleus : P39081
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8720.82

構造登録者

Yang, F.,Varani, G. (登録日: 2016-01-12, 公開日: 2016-11-23, 最終更新日: 2024-05-15)

主引用文献

Yang, F.,Hsu, P.,Lee, S.D.,Yang, W.,Hoskinson, D.,Xu, W.,Moore, C.,Varani, G.
The C terminus of Pcf11 forms a novel zinc-finger structure that plays an essential role in mRNA 3'-end processing.
RNA, 23:98-107, 2017

PubMed Abstract: 3'-End processing of pre-mRNAs prior to packaging and export to the cytoplasm of the mature transcript is a highly regulated process executed by several tens of protein factors that recognize poorly conserved RNA signals. Among them is Pcf11, a highly conserved, multidomain protein that links transcriptional elongation, 3'-end processing, and transcription termination. Here we report the structure and biochemical function of Pcf11's C-terminal domain, which is conserved from yeast to humans. We identify a novel zinc-finger fold, resembling a trillium flower. Structural, biochemical, and genetic analyses reveal a highly conserved surface that plays a critical role in both cleavage and polyadenylation. These findings provide further insight into this important protein and its multiple functional roles during cotranscriptional RNA processing.

PubMed: 27780845
DOI: 10.1261/rna.058354.116

実験手法

SOLUTION NMR