2NAP

DISSIMILATORY NITRATE REDUCTASE (NAP) FROM DESULFOVIBRIO DESULFURICANS

Summary for 2NAP

• Entry DOI: 10.2210/pdb2nap/pdb
• Descriptor: PROTEIN (PERIPLASMIC NITRATE REDUCTASE), IRON/SULFUR CLUSTER, MOLYBDENUM ATOM, ... (6 entities in total)
• Functional Keywords: nitrogenous acceptor, dissimilatory nitrate reductase, multiwavelength anomalous diffraction, molybdopterin cofactor, [4fe-4s] cluster, oxidoreductase
• Biological source: Desulfovibrio desulfuricans
• Total number of polymer chains: 1
• Total formula weight: 82516.75

Authors

Dias, J.M.,Than, M.,Humm, A.,Huber, R.,Bourenkov, G.,Bartunik, H.,Bursakov, S.,Calvete, J.,Caldeira, J.,Carneiro, C.,Moura, J.,Moura, I.,Romao, M.J. (deposition date: 1998-09-18, release date: 1999-09-19, Last modification date: 2023-12-27)

Primary citation

Dias, J.M.,Than, M.E.,Humm, A.,Huber, R.,Bourenkov, G.P.,Bartunik, H.D.,Bursakov, S.,Calvete, J.,Caldeira, J.,Carneiro, C.,Moura, J.J.,Moura, I.,Romao, M.J.
Crystal structure of the first dissimilatory nitrate reductase at 1.9 A solved by MAD methods.
Structure Fold.Des., 7:65-79, 1999

PubMed Abstract: The periplasmic nitrate reductase (NAP) from the sulphate reducing bacterium Desulfovibrio desulfuricans ATCC 27774 is induced by growth on nitrate and catalyses the reduction of nitrate to nitrite for respiration. NAP is a molybdenum-containing enzyme with one bis-molybdopterin guanine dinucleotide (MGD) cofactor and one [4Fe-4S] cluster in a single polypeptide chain of 723 amino acid residues. To date, there is no crystal structure of a nitrate reductase.

PubMed: 10368307
DOI: 10.1016/S0969-2126(99)80010-0

Experimental method

X-RAY DIFFRACTION (1.9 Å)