2N09

NMR structure of a short hydrophobic 11mer peptide in DMSO-d6/H2O (1:3) solution

2N09 の概要

• エントリーDOI: 10.2210/pdb2n09/pdb
• 関連するPDBエントリー: 2N08 2N0I 2N0N
• NMR情報: BMRB: 26536
• 分子名称: Short hydrophobic peptide with cyclic constraints (1 entity in total)
• 機能のキーワード: hydrophobic peptide, glp-1r agonist, de novo protein
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1271.34

構造登録者

Hoang, H.N.,Song, K.,Hill, T.A.,Derksen, D.R.,Edmonds, D.J.,Kok, W.M.,Limberakis, C.,Liras, S.,Loria, P.M.,Mascitti, V.,Mathiowetz, A.M.,Mitchell, J.M.,Piotrowski, D.W.,Price, D.A.,Stanton, R.V.,Suen, J.Y.,Withka, J.M.,Griffith, D.A.,Fairlie, D.P. (登録日: 2015-03-04, 公開日: 2015-04-15, 最終更新日: 2025-03-26)

主引用文献

Hoang, H.N.,Song, K.,Hill, T.A.,Derksen, D.R.,Edmonds, D.J.,Kok, W.M.,Limberakis, C.,Liras, S.,Loria, P.M.,Mascitti, V.,Mathiowetz, A.M.,Mitchell, J.M.,Piotrowski, D.W.,Price, D.A.,Stanton, R.V.,Suen, J.Y.,Withka, J.M.,Griffith, D.A.,Fairlie, D.P.
Short Hydrophobic Peptides with Cyclic Constraints Are Potent Glucagon-like Peptide-1 Receptor (GLP-1R) Agonists.
J.Med.Chem., 58:4080-4085, 2015

PubMed Abstract: Cyclic constraints are incorporated into an 11-residue analogue of the N-terminus of glucagon-like peptide-1 (GLP-1) to investigate effects of structure on agonist activity. Cyclization through linking side chains of residues 2 and 5 or 5 and 9 produced agonists at nM concentrations in a cAMP assay. 2D NMR and CD spectra revealed an N-terminal β-turn and a C-terminal helix that differentially influenced affinity and agonist potency. These structures can inform development of small molecule agonists of the GLP-1 receptor to treat type 2 diabetes.

PubMed: 25839426
DOI: 10.1021/acs.jmedchem.5b00166

実験手法

SOLUTION NMR