2N02

Solution structure of the A147T variant of the mitochondrial translocator protein (tspo) in complex with pk11195

2N02 の概要

• エントリーDOI: 10.2210/pdb2n02/pdb
• 関連するPDBエントリー: 2mgy
• NMR情報: BMRB: 25513
• 分子名称: Translocator protein, N-[(2R)-butan-2-yl]-1-(2-chlorophenyl)-N-methylisoquinoline-3-carboxamide (2 entities in total)
• 機能のキーワード: membrane protein, translocator protein (tspo), protein-ligand complex, mitochondria, pk11195, peripheral benzodiazepine receptor, a147t
• 由来する生物種: Mus musculus (mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19181.57

構造登録者

Jaremko, M.,Jaremko, L.,Giller, K.,Becker, S.,Zweckstetter, M. (登録日: 2015-03-04, 公開日: 2015-06-10, 最終更新日: 2024-05-15)

主引用文献

Jaremko, M.,Jaremko, M.,Giller, K.,Becker, S.,Zweckstetter, M.
Structural Integrity of the A147T Polymorph of Mammalian TSPO.
Chembiochem, 16:1483-1489, 2015

PubMed Abstract: Ligands of the transmembrane protein TSPO are used for imaging of brain inflammation, but a common polymorphism in TSPO complicates their application to humans. Here we determined the three-dimensional structure and side-chain dynamics of the A147T polymorph of mammalian TSPO in complex with the first-generation ligand PK11195. We show that A147T TSPO is able to retain the same structural and dynamic profile as the wild-type protein and thus binds PK11195 with comparable affinity. Our study is important for the design of more potent diagnostic and therapeutic ligands of TSPO.

PubMed: 25974690
DOI: 10.1002/cbic.201500217

実験手法

SOLUTION NMR