2MZP

Structure and dynamics of the acidosis-resistant a162H mutant of the switch region of troponin I bound to the regulatory domain of troponin C

2MZP の概要

• エントリーDOI: 10.2210/pdb2mzp/pdb
• NMR情報: BMRB: 25495
• 分子名称: Troponin C, slow skeletal and cardiac muscles, Troponin I, cardiac muscle, CALCIUM ION (3 entities in total)
• 機能のキーワード: contraction regulation, contraction enhancenment, troponin, acidosis, ca2+ binding, contractile protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13183.02

構造登録者

Pineda Sanabria, S.E.,Robertson, I.M.,Sykes, B.D. (登録日: 2015-02-20, 公開日: 2015-06-03, 最終更新日: 2024-05-15)

主引用文献

Pineda-Sanabria, S.E.,Robertson, I.M.,Sykes, B.D.
Structure and Dynamics of the Acidosis-Resistant A162H Mutant of the Switch Region of Troponin I Bound to the Regulatory Domain of Troponin C.
Biochemistry, 54:3583-3593, 2015

PubMed Abstract: Intracellular acidosis lowers the Ca²⁺ sensitivity of cardiac muscle, which results in decreased force generation, decreased cardiac output, and, eventually, heart failure. The A162H mutant of cardiac troponin I in the thin filament turns the heart acidosis-resistant. Physiological and structural studies have provided insights into the mechanism of protection by the A162H substitution; however, the effect of other native residues of cardiac troponin I is not fully understood. In this study, we determined the structure of the A162H mutant of the switch region of cardiac troponin I bound to the regulatory domain of troponin C at pH 6.1, and the dynamics as a function of pH, by NMR spectroscopy to evaluate the changes induced by protonation of A162H. The results indicate that A162H induces a transitory curved conformation on troponin I that promotes contraction, but it is countered by residue E164 to ensure proper relaxation. Our model explains the absence of diastolic impairment in the gain-of-function phenotype induced by the A162H substitution as well as the effects of a variety of mutants studied previously. The description of this mechanism underlines the fine quality of regulation on cardiac muscle contraction and anticipates pharmacological agents that induce modest changes in the contraction-relaxation equilibrium to produce marked effects in cardiac performance.

PubMed: 25996354
DOI: 10.1021/acs.biochem.5b00178

実験手法

SOLUTION NMR