2MYZ

The Solution Structure of the Magnesium-bound Conantokin-R1B Mutant

2MYZ の概要

• エントリーDOI: 10.2210/pdb2myz/pdb
• 関連するPDBエントリー: 2MZK 2MZL 2MZM
• NMR情報: BMRB: 25465
• 分子名称: Conantokin-R1-B (1 entity in total)
• 機能のキーワード: toxin, nmdar antagonist, metal binding protein, transport protein inhibitor
• 由来する生物種: Conus rolani (Cone snail)
• 細胞内の位置: Secreted : P0DKZ0
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2168.06

構造登録者

Kunda, S.,Yuan, Y.,Balsara, R.D.,Zajicek, J.,Castellino, F.J. (登録日: 2015-02-04, 公開日: 2015-06-17, 最終更新日: 2023-06-14)

主引用文献

Kunda, S.,Yuan, Y.,Balsara, R.D.,Zajicek, J.,Castellino, F.J.
Hydroxyproline-induced Helical Disruption in Conantokin Rl-B Affects Subunit-selective Antagonistic Activities toward Ion Channels of N-Methyl-d-aspartate Receptors.
J.Biol.Chem., 290:18156-18172, 2015

PubMed Abstract: Conantokins are ~20-amino acid peptides present in predatory marine snail venoms that function as allosteric antagonists of ion channels of the N-methyl-d-aspartate receptor (NMDAR). These peptides possess a high percentage of post-/co-translationally modified amino acids, particularly γ-carboxyglutamate (Gla). Appropriately spaced Gla residues allow binding of functional divalent cations, which induces end-to-end α-helices in many conantokins. A smaller number of these peptides additionally contain 4-hydroxyproline (Hyp). Hyp should prevent adoption of the metal ion-induced full α-helix, with unknown functional consequences. To address this disparity, as well as the role of Hyp in conantokins, we have solved the high resolution three-dimensional solution structure of a Gla/Hyp-containing 18-residue conantokin, conRl-B, by high field NMR spectroscopy. We show that Hyp(10) disrupts only a small region of the α-helix of the Mn(2+)·peptide complex, which displays cation-induced α-helices on each terminus of the peptide. The function of conRl-B was examined by measuring its inhibition of NMDA/Gly-mediated current through NMDAR ion channels in mouse cortical neurons. The conRl-B displays high inhibitory selectivity for subclasses of NMDARs that contain the functionally important GluN2B subunit. Replacement of Hyp(10) with N(8)Q results in a Mg(2+)-complexed end-to-end α-helix, accompanied by attenuation of NMDAR inhibitory activity. However, replacement of Hyp(10) with Pro(10) allowed the resulting peptide to retain its inhibitory property but diminished its GluN2B specificity. Thus, these modified amino acids, in specific peptide backbones, play critical roles in their subunit-selective inhibition of NMDAR ion channels, a finding that can be employed to design NMDAR antagonists that function at ion channels of distinct NMDAR subclasses.

PubMed: 26048991
DOI: 10.1074/jbc.M115.650341

実験手法

SOLUTION NMR