2MW6

Structure of the bee venom toxin melittin with [(C5H5)Ru]+ fragment attached to the tryptophan residue

2MW6 の概要

• エントリーDOI: 10.2210/pdb2mw6/pdb
• 関連するPDBエントリー: 2MLT
• NMR情報: BMRB: 25300
• 分子名称: Melittin, [(1,2,3,4,5-eta)-cyclopentadienyl][(1,2,3,4,4a,8a-eta)-naphthalene]ruthenium(1+) (2 entities in total)
• 機能のキーワード: toxin
• 由来する生物種: Apis mellifera (European honey bee,Western honey bee,bee,honeybee)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3144.83

構造登録者

Perekalin, D.S.,Pavlov, A.A.,Novikov, V.V. (登録日: 2014-10-28, 公開日: 2015-07-01, 最終更新日: 2024-11-06)

主引用文献

Perekalin, D.S.,Novikov, V.V.,Pavlov, A.A.,Ivanov, I.A.,Anisimova, N.Y.,Kopylov, A.N.,Volkov, D.S.,Seregina, I.F.,Bolshov, M.A.,Kudinov, A.R.
Selective ruthenium labeling of the tryptophan residue in the bee venom Peptide melittin.
Chemistry, 21:4923-4925, 2015

PubMed Abstract: Melittin is a membrane-active peptide from bee venom with promising antimicrobial and anticancer activity. Herein we report on a simple and selective method for labeling of the tryptophan residue in melittin by the organometallic fragment [(C5 H5 )Ru](+) in aqueous solution and in air. Ruthenium coordination does not disturb the secondary structure of the peptide (as verified by 2D NMR spectroscopy), but changes the pattern of its intermolecular interactions resulting in an 11-fold decrease of hemolytic activity. The high stability of the organometallic conjugate allowed the establishment of the biodistribution of the labeled melittin in mice by inductively coupled plasma MS analysis of ruthenium.

PubMed: 25688543
DOI: 10.1002/chem.201406510

実験手法

SOLUTION NMR