2MOZ

Structure of the Membrane Protein MerF, a Bacterial Mercury Transporter, Improved by the Inclusion of Chemical Shift Anisotropy Constraints

2MOZ の概要

• エントリーDOI: 10.2210/pdb2moz/pdb
• 関連するPDBエントリー: 2M67
• NMR情報: BMRB: 19115
• 分子名称: MerF (1 entity in total)
• 機能のキーワード: membrane protein, membrane protein structures by solution nmr, mpsbynmr, psi-biology, structural genomics
• 由来する生物種: Morganella morganii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8670.40

構造登録者

Tian, Y.,Lu, G.J.,Marassi, F.M.,Opella, S.J.,Membrane Protein Structures by Solution NMR (MPSbyNMR) (登録日: 2014-05-07, 公開日: 2014-07-30, 最終更新日: 2024-05-15)

主引用文献

Tian, Y.,Lu, G.J.,Marassi, F.M.,Opella, S.J.
Structure of the membrane protein MerF, a bacterial mercury transporter, improved by the inclusion of chemical shift anisotropy constraints.
J.Biomol.Nmr, 60:67-71, 2014

PubMed Abstract: MerF is a mercury transport membrane protein from the bacterial mercury detoxification system. By performing a solid-state INEPT experiment and measuring chemical shift anisotropy frequencies in aligned samples, we are able to improve on the accuracy and precision of the initial structure that we presented. MerF has four N-terminal and eleven C-terminal residues that are mobile and unstructured in phospholipid bilayers. The structure presented here has average pairwise RMSDs of 1.78 Å for heavy atoms and 0.92 Å for backbone atoms.

PubMed: 25103921
DOI: 10.1007/s10858-014-9852-0

実験手法

SOLUTION NMR