2MJY

Solution structure of synthetic Mamba-1 peptide

2MJY の概要

• エントリーDOI: 10.2210/pdb2mjy/pdb
• NMR情報: BMRB: 19746
• 分子名称: Mambalgin-1 (1 entity in total)
• 機能のキーワード: toxin, manba
• 由来する生物種: Dendroaspis polylepis polylepis (Black mamba)
• 細胞内の位置: Secreted : P0DKR6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6574.64

構造登録者

He, Y.,Wu, F.,Tian, C. (登録日: 2014-01-21, 公開日: 2015-01-28, 最終更新日: 2024-11-06)

主引用文献

Pan, M.,He, Y.,Wen, M.,Wu, F.,Sun, D.,Li, S.,Zhang, L.,Li, Y.,Tian, C.
One-pot hydrazide-based native chemical ligation for efficient chemical synthesis and structure determination of toxin Mambalgin-1
Chem.Commun.(Camb.), 50:5837-5839, 2014

PubMed Abstract: An efficient one-pot chemical synthesis of snake venom toxin Mambalgin-1 was achieved using an azide-switch strategy combined with hydrazide-based native chemical ligation. Synthetic Mambalgin-1 exhibited a well-defined structure after sequential folding in vitro. NMR spectroscopy revealed a three-finger toxin family structure, and the synthetic toxin inhibited human acid-sensing ion channel 1a.

PubMed: 24619065
DOI: 10.1039/c4cc00779d

実験手法

SOLUTION NMR