2MI8

Solution structure of lysine-free (K0) ubiquitin

2MI8 の概要

• エントリーDOI: 10.2210/pdb2mi8/pdb
• NMR情報: BMRB: 19670
• 分子名称: Ubiquitin (1 entity in total)
• 機能のキーワード: signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Ubiquitin: Cytoplasm (By similarity): P0CG48
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8772.93

構造登録者

Huang, T.,Li, J.R.,Byrd, A.R. (登録日: 2013-12-09, 公開日: 2014-04-02, 最終更新日: 2024-05-15)

主引用文献

Huang, T.,Li, J.,Byrd, R.A.
Solution structure of lysine-free (K0) ubiquitin.
Protein Sci., 23:662-667, 2014

PubMed Abstract: Lysine-free ubiquitin (K0-Ub) is commonly used to study the ubiquitin-signaling pathway, where it is assumed to have the same structure and function as wild-type ubiquitin (wt-Ub). However, the K0-Ub (15) N heteronuclear single quantum correlation NMR spectrum differs significantly from wt-Ub and the melting temperature is depressed by 19°C, raising the question of the structural integrity and equivalence to wt-Ub. The three-dimensional structure of K0-Ub was determined by solution NMR, using chemical shift and residual dipolar coupling data. K0-Ub adopts the same backbone structure as wt-Ub, and all significant chemical shifts can be related to interactions impacted by the K to R mutations.

PubMed: 24591328
DOI: 10.1002/pro.2450

実験手法

SOLUTION NMR