2MEM

Solution NMR structure of SLED domain of Scml2

2MEM の概要

• エントリーDOI: 10.2210/pdb2mem/pdb
• 関連するPDBエントリー: 1OI1 2BIV 2VYT 4EDU
• NMR情報: BMRB: 19526
• 分子名称: Sex comb on midleg-like protein 2 (1 entity in total)
• 機能のキーワード: prc1, pcg, transcription
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus (Probable): Q9UQR0
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13112.89

構造登録者

Bezsonova, I. (登録日: 2013-09-24, 公開日: 2014-04-23, 最終更新日: 2024-05-15)

主引用文献

Bezsonova, I.
Solution NMR Structure of the DNA-binding Domain from Scml2 (Sex Comb on Midleg-like 2).
J.Biol.Chem., 289:15739-15749, 2014

PubMed Abstract: Scml2 is a member of the Polycomb group of proteins involved in epigenetic gene silencing. Human Scml2 is a part of a multisubunit protein complex, PRC1 (Polycomb repressive complex 1), which is responsible for maintenance of gene repression, prevention of chromatin remodeling, preservation of the "stemness" of the cell, and cell differentiation. Although the majority of PRC1 subunits have been recently characterized, the structure of Scml2 and its role in PRC1-mediated gene silencing remain unknown. In this work a conserved protein domain within human Scml2 has been identified, and its structure was determined by solution NMR spectroscopy. This module was named Scm-like embedded domain, or SLED. Evolutionarily, the SLED domain emerges in the first multicellular organisms, consistent with the role of Scml2 in cell differentiation. Furthermore, it is exclusively found within the Scm-like family of proteins, often accompanied by malignant brain tumor domain (MBT) and sterile α motif (SAM) domains. The domain adopts a novel α/β fold with no structural analogues found in the Protein Data Bank (PDB). The ability of the SLED to bind double-stranded DNA was also examined, and the isolated domain was shown to interact with DNA in a sequence-specific manner. Because PRC1 complexes localize to the promoters of a specific subset of developmental genes in vivo, the SLED domain of Scml2 may provide an important link connecting the PRC1 complexes to their target genes.

PubMed: 24727478
DOI: 10.1074/jbc.M113.524009

実験手法

SOLUTION NMR