2ME1

HIV-1 gp41 clade B double alanine mutant Membrane Proximal External Region peptide in DPC micelle

Summary for 2ME1

• Entry DOI: 10.2210/pdb2me1/pdb
• Related: 2ME2 2ME3 2ME4 2PV6
• NMR Information: BMRB: 19512
• Descriptor: Gp41 (1 entity in total)
• Functional Keywords: mper, viral fusion, helix-hinge-helix, membrane protein
• Biological source: Human immunodeficiency virus 1
• Cellular location: Transmembrane protein gp41: Virion membrane; Single-pass type I membrane protein. Surface protein gp120: Virion membrane; Peripheral membrane protein: P04578
• Total number of polymer chains: 1
• Total formula weight: 3455.95

Authors

Sun, Z.J.,Wagner, G.,Reinherz, E.L.,Kim, M.,Song, L.,Choi, J.,Cheng, Y.,Chowdhury, B.,Bellot, G.,Shih, W. (deposition date: 2013-09-20, release date: 2013-10-09, Last modification date: 2024-05-15)

Primary citation

Sun, Z.Y.,Cheng, Y.,Kim, M.,Song, L.,Choi, J.,Kudahl, U.J.,Brusic, V.,Chowdhury, B.,Yu, L.,Seaman, M.S.,Bellot, G.,Shih, W.M.,Wagner, G.,Reinherz, E.L.
Disruption of Helix-Capping Residues 671 and 674 Reveals a Role in HIV-1 Entry for a Specialized Hinge Segment of the Membrane Proximal External Region of gp41.
J.Mol.Biol., 426:1095-1108, 2014

PubMed Abstract: HIV-1 (human immunodeficiency virus type 1) uses its trimeric gp160 envelope (Env) protein consisting of non-covalently associated gp120 and gp41 subunits to mediate entry into human T lymphocytes. A facile virus fusion mechanism compensates for the sparse Env copy number observed on viral particles and includes a 22-amino-acid, lentivirus-specific adaptation at the gp41 base (amino acid residues 662-683), termed the membrane proximal external region (MPER). We show by NMR and EPR that the MPER consists of a structurally conserved pair of viral lipid-immersed helices separated by a hinge with tandem joints that can be locked by capping residues between helices. This design fosters efficient HIV-1 fusion via interconverting structures while, at the same time, affording immune escape. Disruption of both joints by double alanine mutations at Env positions 671 and 674 (AA) results in attenuation of Env-mediated cell-cell fusion and hemifusion, as well as viral infectivity mediated by both CD4-dependent and CD4-independent viruses. The potential mechanism of disruption was revealed by structural analysis of MPER conformational changes induced by AA mutation. A deeper acyl chain-buried MPER middle section and the elimination of cross-hinge rigid-body motion almost certainly impede requisite structural rearrangements during the fusion process, explaining the absence of MPER AA variants among all known naturally occurring HIV-1 viral sequences. Furthermore, those broadly neutralization antibodies directed against the HIV-1 MPER exploit the tandem joint architecture involving helix capping, thereby disrupting hinge function.

PubMed: 24075869
DOI: 10.1016/j.jmb.2013.09.030

Experimental method

SOLUTION NMR