2MB2

parallel-stranded G-quadruplex in DNA poly-G stretches

2MB2 の概要

• エントリーDOI: 10.2210/pdb2mb2/pdb
• NMR情報: BMRB: 19386
• 分子名称: DNA_(5'-D(*TP*TP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*T)-3') (1 entity in total)
• 機能のキーワード: g-tracts, g-quadruplex, propeller structure, poly-g stretches, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5805.71

構造登録者

Sengar, A.,Heddi, B.,Phan, A.T. (登録日: 2013-07-24, 公開日: 2014-12-03, 最終更新日: 2024-05-15)

主引用文献

Sengar, A.,Heddi, B.,Phan, A.T.
Formation of g-quadruplexes in poly-g sequences: structure of a propeller-type parallel-stranded g-quadruplex formed by a g15 stretch.
Biochemistry, 53:7718-7723, 2014

PubMed Abstract: Poly-G sequences are found in different genomes including human and have the potential to form higher-order structures with various applications. Previously, long poly-G sequences were thought to lead to multiple possible ways of G-quadruplex folding, rendering their structural characterization challenging. Here we investigate the structure of G-quadruplexes formed by poly-G sequences d(TTG(n)T), where n = 12 to 19. Our data show the presence of multiple and/or higher-order G-quadruplex structures in most sequences. Strikingly, NMR spectra of the TTG₁₅T sequence containing a stretch of 15 continuous guanines are exceptionally well-resolved and indicate the formation of a well-defined G-quadruplex structure. The NMR solution structure of this sequence revealed a propeller-type parallel-stranded G-quadruplex containing three G-tetrad layers and three single-guanine propeller loops. The same structure can potentially form anywhere along a long G(n) stretch, making it unique for molecular recognition by other cellular molecules.

PubMed: 25375976
DOI: 10.1021/bi500990v

実験手法

SOLUTION NMR