2M8M

NMR assignment and structure of a peptide derived from the membrane proximal external region of HIV-1 gp41 in the presence of hexafluoroisopropanol

2M8M の概要

• エントリーDOI: 10.2210/pdb2m8m/pdb
• 関連するPDBエントリー: 2M8O
• NMR情報: BMRB: 19262
• 分子名称: Transmembrane protein gp41 (1 entity in total)
• 機能のキーワード: hiv-1 gp41 protein, neutralizing epitope, peptide vaccine, viral protein
• 由来する生物種: Human immunodeficiency virus type 1 (HIV-1)
• 細胞内の位置: Transmembrane protein gp41: Virion membrane; Single-pass type I membrane protein. Surface protein gp120: Virion membrane; Peripheral membrane protein: P05878
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3656.11

構造登録者

Serrano, S.,Huarte, N.,Nieva, J.L.,Jimenez, M. (登録日: 2013-05-23, 公開日: 2014-01-22, 最終更新日: 2024-05-15)

主引用文献

Serrano, S.,Araujo, A.,Apellaniz, B.,Bryson, S.,Carravilla, P.,de la Arada, I.,Huarte, N.,Rujas, E.,Pai, E.F.,Arrondo, J.L.,Domene, C.,Jimenez, M.A.,Nieva, J.L.
Structure and Immunogenicity of a Peptide Vaccine, Including the Complete HIV-1 gp41 2F5 Epitope: IMPLICATIONS FOR ANTIBODY RECOGNITION MECHANISM AND IMMUNOGEN DESIGN.
J.Biol.Chem., 289:6565-6580, 2014

PubMed Abstract: The membrane-proximal external region (MPER) of gp41 harbors the epitope recognized by the broadly neutralizing anti-HIV 2F5 antibody, a research focus in HIV-1 vaccine development. In this work, we analyze the structure and immunogenic properties of MPERp, a peptide vaccine that includes the following: (i) the complete sequence protected from proteolysis by the 2F5 paratope; (ii) downstream residues postulated to establish weak contacts with the CDR-H3 loop of the antibody, which are believed to be crucial for neutralization; and (iii) an aromatic rich anchor to the membrane interface. MPERp structures solved in dodecylphosphocholine micelles and 25% 1,1,1,3,3,3-hexafluoro-2-propanol (v/v) confirmed folding of the complete 2F5 epitope within continuous kinked helices. Infrared spectroscopy (IR) measurements demonstrated the retention of main helical conformations in immunogenic formulations based on alum, Freund's adjuvant, or two different types of liposomes. Binding to membrane-inserted MPERp, IR, molecular dynamics simulations, and characterization of the immune responses further suggested that packed helical bundles partially inserted into the lipid bilayer, rather than monomeric helices adsorbed to the membrane interface, could encompass effective MPER peptide vaccines. Together, our data constitute a proof-of-concept to support MPER-based peptides in combination with liposomes as stand-alone immunogens and suggest new approaches for structure-aided MPER vaccine development.

PubMed: 24429284
DOI: 10.1074/jbc.M113.527747

実験手法

SOLUTION NMR