2M7A

Enteropathogenic Escherichia coli 0111:H- str. 11128 ORF EC0111_1119 similar to bacteriophage lambda ea8.5

2M7A の概要

• エントリーDOI: 10.2210/pdb2m7a/pdb
• 関連するPDBエントリー: 2M7B
• NMR情報: BMRB: 19178
• 分子名称: Uncharacterized protein, ZINC ION (2 entities in total)
• 機能のキーワード: bacteriophage, zinc binding, chcc motif, unknown function
• 由来する生物種: Escherichia coli (Escherichia coli)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11391.98

構造登録者

Donaldson, L.W.F. (登録日: 2013-04-18, 公開日: 2013-06-12, 最終更新日: 2024-05-01)

主引用文献

Kwan, J.J.,Smirnova, E.,Khazai, S.,Evanics, F.,Maxwell, K.L.,Donaldson, L.W.
The Solution Structures of Two Prophage Homologues of the Bacteriophage lambda Ea8.5 Protein Reveal a Newly Discovered Hybrid Homeodomain/Zinc-Finger Fold.
Biochemistry, 52:3612-3614, 2013

PubMed Abstract: A cluster of genes in the exoxis region of bacteriophage λ are capable of inhibiting the initiation of DNA synthesis in Escherichia coli. The most indispensible gene in this region is ea8.5. Here, we report the nuclear magnetic resonance structures of two ea8.5 orthologs from enteropathogenic E. coli and Pseudomonas putida prophages. Both proteins are characterized by a fused homeodomain/zinc-finger fold that escaped detection by primary sequence search methods. While these folds are both associated with a nucleic acid binding function, the amino acid composition suggests otherwise, leading to the possibility that Ea8.5 associates with other viral and host proteins.

PubMed: 23672713
DOI: 10.1021/bi400543w

実験手法

SOLUTION NMR