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2M6P

The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase

2M6P の概要
エントリーDOI10.2210/pdb2m6p/pdb
関連するPDBエントリー2M6O
NMR情報BMRB: 19149
分子名称uncharacterized protein Mb2076 (1 entity in total)
機能のキーワードmv2050, mtb, rnap, sigma factor, transcription
由来する生物種Mycobacterium bovis
タンパク質・核酸の鎖数1
化学式量合計5213.68
構造登録者
Liu, B.,Parsy, M.,Paget, M.,Matthews, S. (登録日: 2013-04-06, 公開日: 2013-05-08, 最終更新日: 2024-05-01)
主引用文献Tabib-Salazar, A.,Liu, B.,Doughty, P.,Lewis, R.A.,Ghosh, S.,Parsy, M.L.,Simpson, P.J.,O'Dwyer, K.,Matthews, S.J.,Paget, M.S.
The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase.
Nucleic Acids Res., 41:5679-5691, 2013
Cited by
PubMed Abstract: RbpA is a small non-DNA-binding transcription factor that associates with RNA polymerase holoenzyme and stimulates transcription in actinobacteria, including Streptomyces coelicolor and Mycobacterium tuberculosis. RbpA seems to show specificity for the vegetative form of RNA polymerase as opposed to alternative forms of the enzyme. Here, we explain the basis of this specificity by showing that RbpA binds directly to the principal σ subunit in these organisms, but not to more diverged alternative σ factors. Nuclear magnetic resonance spectroscopy revealed that, although differing in their requirement for structural zinc, the RbpA orthologues from S. coelicolor and M. tuberculosis share a common structural core domain, with extensive, apparently disordered, N- and C-terminal regions. The RbpA-σ interaction is mediated by the C-terminal region of RbpA and σ domain 2, and S. coelicolor RbpA mutants that are defective in binding σ are unable to stimulate transcription in vitro and are inactive in vivo. Given that RbpA is essential in M. tuberculosis and critical for growth in S. coelicolor, these data support a model in which RbpA plays a key role in the σ cycle in actinobacteria.
PubMed: 23605043
DOI: 10.1093/nar/gkt277
主引用文献が同じPDBエントリー
実験手法
SOLUTION NMR
構造検証レポート
Validation report summary of 2m6p
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-09に公開中

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