2M6P
The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase
2M6P の概要
エントリーDOI | 10.2210/pdb2m6p/pdb |
関連するPDBエントリー | 2M6O |
NMR情報 | BMRB: 19149 |
分子名称 | uncharacterized protein Mb2076 (1 entity in total) |
機能のキーワード | mv2050, mtb, rnap, sigma factor, transcription |
由来する生物種 | Mycobacterium bovis |
タンパク質・核酸の鎖数 | 1 |
化学式量合計 | 5213.68 |
構造登録者 | |
主引用文献 | Tabib-Salazar, A.,Liu, B.,Doughty, P.,Lewis, R.A.,Ghosh, S.,Parsy, M.L.,Simpson, P.J.,O'Dwyer, K.,Matthews, S.J.,Paget, M.S. The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase. Nucleic Acids Res., 41:5679-5691, 2013 Cited by PubMed Abstract: RbpA is a small non-DNA-binding transcription factor that associates with RNA polymerase holoenzyme and stimulates transcription in actinobacteria, including Streptomyces coelicolor and Mycobacterium tuberculosis. RbpA seems to show specificity for the vegetative form of RNA polymerase as opposed to alternative forms of the enzyme. Here, we explain the basis of this specificity by showing that RbpA binds directly to the principal σ subunit in these organisms, but not to more diverged alternative σ factors. Nuclear magnetic resonance spectroscopy revealed that, although differing in their requirement for structural zinc, the RbpA orthologues from S. coelicolor and M. tuberculosis share a common structural core domain, with extensive, apparently disordered, N- and C-terminal regions. The RbpA-σ interaction is mediated by the C-terminal region of RbpA and σ domain 2, and S. coelicolor RbpA mutants that are defective in binding σ are unable to stimulate transcription in vitro and are inactive in vivo. Given that RbpA is essential in M. tuberculosis and critical for growth in S. coelicolor, these data support a model in which RbpA plays a key role in the σ cycle in actinobacteria. PubMed: 23605043DOI: 10.1093/nar/gkt277 主引用文献が同じPDBエントリー |
実験手法 | SOLUTION NMR |
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