2M3S

Calmodulin, i85l, f92e, h107i, l112r, a128t, m144r mutant

2M3S の概要

• エントリーDOI: 10.2210/pdb2m3s/pdb
• 関連するPDBエントリー: 2KZ2
• NMR情報: BMRB: 18976
• 分子名称: Calmodulin, CALCIUM ION (2 entities in total)
• 機能のキーワード: calmodulin, metal binding protein
• 由来する生物種: Gallus gallus (chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17270.10

構造登録者

Moroz, Y.S.,Wu, Y.,Cheng, H.,Roder, H.,Korendovych, I.V. (登録日: 2013-01-25, 公開日: 2013-07-24, 最終更新日: 2024-05-15)

主引用文献

Moroz, O.V.,Moroz, Y.S.,Wu, Y.,Olsen, A.B.,Cheng, H.,Mack, K.L.,McLaughlin, J.M.,Raymond, E.A.,Zhezherya, K.,Roder, H.,Korendovych, I.V.
A single mutation in a regulatory protein produces evolvable allosterically regulated catalyst of nonnatural reaction.
Angew.Chem.Int.Ed.Engl., 52:6246-6249, 2013

PubMed Abstract: a strategically placed single mutation in a non-enzymatic protein scaffold produced AlleyCat, a small, allosterically regulated catalyst of Kemp elimination. In only 7 rounds of directed evolution enzymatic efficiency of the original 74 amino acid residue catalyst was improved more than 220-fold to achieve k value higher than that of catalytic antibodies for the same reaction, still preserving allosteric regulation.

PubMed: 23630096
DOI: 10.1002/anie.201302339

実験手法

SOLUTION NMR