2LVN

Structure of the gp78 CUE domain

2LVN の概要

• エントリーDOI: 10.2210/pdb2lvn/pdb
• 関連するPDBエントリー: 2LVO 2LVP 2LVQ
• NMR情報: BMRB: 18581
• 分子名称: E3 ubiquitin-protein ligase AMFR (1 entity in total)
• 機能のキーワード: cue domain, ligase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Endoplasmic reticulum membrane; Multi-pass membrane protein: Q9UKV5
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5966.75

構造登録者

Liu, S.,Chen, Y.,Huang, T.,Tarasov, S.G.,King, A.,Li, J.,Weissman, A.M.,Byrd, R.A.,Das, R. (登録日: 2012-07-09, 公開日: 2012-11-21, 最終更新日: 2024-05-01)

主引用文献

Liu, S.,Chen, Y.,Li, J.,Huang, T.,Tarasov, S.,King, A.,Weissman, A.M.,Byrd, R.A.,Das, R.
Promiscuous Interactions of gp78 E3 Ligase CUE Domain with Polyubiquitin Chains.
Structure, 20:2138-2150, 2012

PubMed Abstract: Recognition of ubiquitin and polyubiquitin chains by ubiquitin-binding domains (UBDs) is vital for ubiquitin-mediated signaling pathways. The endoplasmic reticulum resident RING finger ubiquitin ligase (E3) gp78 regulates critical proteins via the ubiquitin-proteasome system to maintain cellular homeostasis and includes a UBD known as the CUE domain, which is essential for function. A probable role of this domain is to recognize ubiquitin-modified substrates, enabling gp78 to assemble polyubiquitin chains on these substrates and mark them for degradation. Here, we report the molecular details of the interaction of gp78CUE domain with ubiquitin and diubiquitin. The gp78CUE domain exhibits a well-defined set of interactions with ubiquitin and a dynamic, promiscuous interaction with diubiquitin chains. This leads to a model in which the CUE domain functions to both facilitate substrate binding and enable switching between adjacent ubiquitin molecules of a growing chain to enable processivity in ubiquitination.

PubMed: 23123110
DOI: 10.1016/j.str.2012.09.020

実験手法

SOLUTION NMR