2LTZ

Smurf2 WW3 domain in complex with a Smad7 derived peptide

2LTZ の概要

• エントリーDOI: 10.2210/pdb2ltz/pdb
• 関連するPDBエントリー: 2LTV 2LTW 2LTX 2LTY
• NMR情報: BMRB: 18502
• 分子名称: E3 ubiquitin-protein ligase SMURF2, Smad7 derived peptide (2 entities in total)
• 機能のキーワード: ww, smurf2, smad7, protein binding-peptide complex, protein binding/peptide
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: Q9HAU4 O15105
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5983.55

構造登録者

Macias, M.J.,Aragon, E.,Goerner, N.,Xi, Q.,Lopes, T.,Gao, S.,Massague, J. (登録日: 2012-06-04, 公開日: 2012-11-21, 最終更新日: 2024-05-01)

主引用文献

Aragon, E.,Goerner, N.,Xi, Q.,Gomes, T.,Gao, S.,Massague, J.,Macias, M.J.
Structural Basis for the Versatile Interactions of Smad7 with Regulator WW Domains in TGF-beta Pathways.
Structure, 20:1726-1736, 2012

PubMed Abstract: Transforming growth factor (TGF)-β and BMP signaling is mediated by Smads 1-5 (R-Smads and Co-Smads) and inhibited by Smad7, a major hub of regulation of TGF-β and BMP receptors by negative feedback and antagonistic signals. The transcription coactivator YAP and the E3 ubiquitin ligases Smurf1/2 and Nedd4L target R-Smads for activation or degradation, respectively. Pairs of WW domain in these regulators bind PY motifs and adjacent CDK/MAPK and GSK3 phosphorylation sites in R-Smads in a selective and regulated manner. In contrast, here we show that Smad7 binds YAP, Smurf1, Smurf2, and Nedd4L constitutively, the binding involving a PY motif in Smad7 and no phosphorylation. We also provide a structural basis for how regulators that use WW domain pairs for selective interactions with R-Smads, resort to one single versatile WW domain for binding Smad7 to centralize regulation in the TGF-β and BMP pathways.

PubMed: 22921829
DOI: 10.1016/j.str.2012.07.014

実験手法

SOLUTION NMR