2LNE

Neurotensin 40 structures in water pH 5.5 298 K. NMR data & structures

Summary for 2LNE

• Entry DOI: 10.2210/pdb2lne/pdb
• Related: 2LNF 2LNG
• NMR Information: BMRB: 18162
• Descriptor: Neurotensin (1 entity in total)
• Functional Keywords: neurotensin(nt), recombinant technology, neuropeptide
• Biological source: Homo sapiens (human)
• Cellular location: Secreted: P30990
• Total number of polymer chains: 1
• Total formula weight: 1693.96

Authors

Mukhopadhyay, C.,Khatun, U. (deposition date: 2011-12-27, release date: 2012-11-14, Last modification date: 2024-05-15)

Primary citation

Khatun, U.L.,Goswami, S.K.,Mukhopadhyay, C.
Modulation of the neurotensin solution structure in the presence of ganglioside GM1 bicelle.
Biophys.Chem., 168:48-59, 2012

PubMed Abstract: Neurotensin (NT) is an endogenous tridecapeptide neurotransmitter that shows multiple biological function in central and peripheral nervous systems. Gangliosides are glycosphingolipids, most abundant in the plasma membrane of nerve cells. Here we investigate the change of neurotensin solution structure induced by isotropic CHAPS-PC bicelles with and without ganglioside GM1 using solution state NMR spectroscopy. In aqueous solution the peptide is predominately unstructured. In the presence of bicelle overall structure of the peptide is stabilized. In CHAPS-PC bicelle neurotensin adopts 3(10) helical structure. In the presence of GM1 containing bicelle, the peptide adopts predominately 3(10) helical structures with small amount of α-helical structure. These results are consistent with the CD spectroscopic results. Neurotensin interacts better with GM1 containing bicelle than that of the CHAPS-PC bicelle. Docking studies between the Neurotensin Receptor3 (NTS3) and different NT conformations also indicated better binding of the NT conformation obtained in presence of GM1-containing bicelles.

PubMed: 22824320
DOI: 10.1016/j.bpc.2012.06.003

Experimental method

SOLUTION NMR