2LM9

The NMR structure of a major allergen from dust mite

2LM9 の概要

• エントリーDOI: 10.2210/pdb2lm9/pdb
• NMR情報: BMRB: 18107
• 分子名称: Blo t 21 allergen (1 entity in total)
• 機能のキーワード: dust mite, allergen
• 由来する生物種: Blomia tropicalis (Mite)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11208.64

構造登録者

Tan, K.W.,Mok, Y.K. (登録日: 2011-11-29, 公開日: 2012-08-22, 最終更新日: 2024-05-15)

主引用文献

Tan, K.W.,Ong, T.C.,Gao, Y.F.,Tiong, Y.S.,Wong, K.N.,Chew, F.T.,Mok, Y.K.
NMR structure and IgE epitopes of Blo t 21, a major dust mite allergen from Blomia tropicalis
J.Biol.Chem., 2012

PubMed Abstract: Blo t 21 is a paralogue of the group 5 allergen, Blo t 5, a major allergen from the dust mite Blomia tropicalis. Blo t 21 has moderate sequence identity (40.7%) to Blo t 5 and low to moderate cross-reactivity to Blo t 5. In B. tropicalis, the most prevalent and allergenic allergens are in the order of Blo t 21, Blo t 5, and Blo t 7. Here, we determined the NMR solution structure of Blo t 21, which represents the first structure of the group 21 dust mite allergen. The structure of Blo t 21 closely resembles the structures of Blo t 5 and Der p 5, comprising three anti-parallel α-helices arranged in a helical bundle. Using site-directed mutagenesis and specific IgE binding ELISA, Blo t 21 was found to contain both conserved and unique charged IgE epitope residues at the L2 loop region and on helix α3. Cross-inhibition assays confirmed that Blo t 21 has a low to moderate cross-reactivity with Blo t 5 and Der p 5 and represents a novel group of major allergen in B. tropicalis. In addition to group 5 allergens, Blo t 21 has also a low to moderate cross-reactivity with group 21 allergens from Dermatophagoides mites, confirming that B. tropicalis is a major and distinct source of dust mite allergens.

PubMed: 22887997
DOI: 10.1074/jbc.M112.348730

実験手法

SOLUTION NMR