2LKF

LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS

2LKF の概要

• エントリーDOI: 10.2210/pdb2lkf/pdb
• 分子名称: LEUKOCIDIN F SUBUNIT (2 entities in total)
• 機能のキーワード: leukotoxin, hemolysin, pore-forming toxin, toxin
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34090.72

構造登録者

Olson, R.,Nariya, H.,Yokota, K.,Kamio, Y.,Gouaux, J.E. (登録日: 1998-07-28, 公開日: 1999-07-28, 最終更新日: 2024-04-03)

主引用文献

Olson, R.,Nariya, H.,Yokota, K.,Kamio, Y.,Gouaux, E.
Crystal structure of staphylococcal LukF delineates conformational changes accompanying formation of a transmembrane channel.
Nat.Struct.Biol., 6:134-140, 1999

PubMed Abstract: Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are self-assembling, channel-forming proteins related in sequence and function. In the alpha-hemolysin heptamer, the channel-forming beta-strands and the amino latch make long excursions from the protomer core. Here we report the crystal structure of the water soluble form of LukF. In the LukF structure the channel-forming region folds into an amphipathic, three-strand beta-sheet and the amino latch forms a beta-strand extending a central beta-sheet. The LukF structure illustrates how a channel-forming toxin masks protein-protein and protein-membrane interfaces prior to cell binding and assembly, and together with the alpha-hemolysin heptamer structure, they define the end points on the pathway of toxin assembly.

PubMed: 10048924
DOI: 10.1038/5821

実験手法

X-RAY DIFFRACTION (2.5 Å)