2LK7

Monomer-dimer equilibrium for 5'-5' stacking of propeller-type parallel-stranded G-quadruplexes: NMR structural study

2LK7 の概要

• エントリーDOI: 10.2210/pdb2lk7/pdb
• NMR情報: BMRB: 17980
• 分子名称: DNA (5'-D(*TP*TP*GP*GP*GP*TP*GP*GP*GP*TP*GP*GP*GP*TP*GP*GP*GP*T)-3') (1 entity in total)
• 機能のキーワード: dna, g-quadruplex, propeller type, monomer
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5730.67

構造登録者

Do, N.Q.,Phan, A.T. (登録日: 2011-10-07, 公開日: 2012-10-10, 最終更新日: 2024-05-15)

主引用文献

Do, N.Q.,Phan, A.T.
Monomer-Dimer Equilibrium for the 5'-5' Stacking of Propeller-Type Parallel-Stranded G-Quadruplexes: NMR Structural Study
Chemistry, 2012

PubMed Abstract: Guanine-rich sequence motifs, which contain tracts of three consecutive guanines connected by single non-guanine nucleotides, are abundant in the human genome and can form a robust G-quadruplex structure with high stability. Herein, by using NMR spectroscopy, we investigate the equilibrium between monomeric and 5'-5' stacked dimeric propeller-type G-quadruplexes that are formed by DNA sequences containing GGGT motifs. We show that the monomer-dimer equilibrium depends on a number of parameters, including the DNA concentration, DNA flanking sequences, the concentration and type of cations, and the temperature. We report on the high-definition structure of a simple monomeric G-quadruplex containing three single-residue loops, which could serve as a reference for propeller-type G-quadruplex structures in solution.

PubMed: 23019076
DOI: 10.1002/chem.201103295

実験手法

SOLUTION NMR