2LF1

Solution structure of L. casei dihydrofolate reductase complexed with NADPH, 30 structures

Summary for 2LF1

• Entry DOI: 10.2210/pdb2lf1/pdb
• Related: 1LUD 2HM9 2L28
• NMR Information: BMRB: 17311
• Descriptor: Dihydrofolate reductase, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (2 entities in total)
• Functional Keywords: oxidoreductase, dhfr, positive cooperativity, protein-ligand interactions
• Biological source: Lactobacillus casei
• Total number of polymer chains: 1
• Total formula weight: 19077.01

Authors

Polshakov, V.,Feeney, J.,Birdsall, B.,Kovalevskaya, N. (deposition date: 2011-06-28, release date: 2011-12-21)

• Primary citation: Feeney, J.,Birdsall, B.,Kovalevskaya, N.V.,Smurnyy, Y.D.,Navarro Peran, E.M.,Polshakov, V.I.; NMR structures of apo L. casei dihydrofolate reductase and its complexes with trimethoprim and NADPH: contributions to positive cooperative binding from ligand-induced refolding, conformational changes, and interligand hydrophobic interactions; Biochemistry, 50:3609-3620, 2011; PubMed: 21410224

Experimental method

SOLUTION NMR