2LE6

Structure of a dimeric all-parallel-stranded G-quadruplex stacked via the 5'-to-5' interface

2LE6 の概要

• エントリーDOI: 10.2210/pdb2le6/pdb
• 関連するPDBエントリー: 1Y8D
• NMR情報: BMRB: 17697
• 分子名称: DNA (5'-D(*GP*IP*GP*TP*GP*GP*GP*TP*GP*GP*GP*TP*GP*GP*GP*T)-3') (1 entity in total)
• 機能のキーワード: hiv-1 integrase inhibition, g-quadruplex, anticancer, stacking, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 10214.54

構造登録者

Do, N.Q.,Lim, K.W.,Teo, M.H.,Heddi, B.,Phan, A.T. (登録日: 2011-06-10, 公開日: 2011-08-31, 最終更新日: 2024-05-15)

主引用文献

Do, N.Q.,Lim, K.W.,Teo, M.H.,Heddi, B.,Phan, A.T.
Stacking of G-quadruplexes: NMR structure of a G-rich oligonucleotide with potential anti-HIV and anticancer activity
Nucleic Acids Res., 2011

PubMed Abstract: G-rich oligonucleotides T30695 (or T30923), with the sequence of (GGGT)(4), and T40214, with the sequence of (GGGC)(4), have been reported to exhibit anti-HIV and anticancer activity. Here we report on the structure of a dimeric G-quadruplex adopted by a derivative of these sequences in K(+) solution. It comprises two identical propeller-type parallel-stranded G-quadruplex subunits each containing three G-tetrad layers that are stacked via the 5'-5' interface. We demonstrated control over the stacking of the two monomeric subunits by sequence modifications. Our analysis of possible structures at the stacking interface provides a general principle for stacking of G-quadruplexes, which could have implications for the assembly and recognition of higher-order G-quadruplex structures.

PubMed: 21840903
DOI: 10.1093/nar/gkr539

実験手法

SOLUTION NMR