2LAG

Structure of the 44 kDa complex of interferon-alpha2 with the extracellular part of IFNAR2 obtained by 2D-double difference NOESY

2LAG の概要

• エントリーDOI: 10.2210/pdb2lag/pdb
• 関連するPDBエントリー: 1kz1
• NMR情報: BMRB: 16677
• 分子名称: Interferon alpha/beta receptor 2, Interferon alpha-2 (2 entities in total)
• 機能のキーワード: interferon, receptor, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Secreted: P48551; Isoform 1: Membrane; Single-pass type I membrane protein. Isoform 2: Membrane; Single-pass type I membrane protein. Isoform 3: Secreted: P01563
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43587.53

構造登録者

Nudelman, I.,Akabayov, S.R.,Scherf, T.,Anglister, J. (登録日: 2011-03-13, 公開日: 2011-08-17, 最終更新日: 2024-10-30)

主引用文献

Nudelman, I.,Akabayov, S.R.,Scherf, T.,Anglister, J.
Observation of Intermolecular Interactions in Large Protein Complexes by 2D-Double Difference Nuclear Overhauser Enhancement Spectroscopy: Application to the 44 kDa Interferon-Receptor Complex.
J.Am.Chem.Soc., 133:14755-14764, 2011

PubMed Abstract: NMR detection of intermolecular interactions between protons in large protein complexes is very challenging because it is difficult to distinguish between weak NOEs from intermolecular interactions and the much larger number of strong intramolecular NOEs. This challenging task is exacerbated by the decrease in signal-to-noise ratio in the often used isotope-edited and isotope-filtered experiments as a result of enhanced T(2) relaxation. Here, we calculate a double difference spectrum that shows exclusively intermolecular NOEs and manifests the good signal-to-noise ratio in 2D homonuclear NOESY spectra even for large proteins. The method is straightforward and results in a complete picture of all intermolecular interactions involving non exchangeable protons. Ninety-seven such (1)H-(1)H NOEs were assigned for the 44 KDa interferon-α2/IFNAR2 complex and used for docking these two proteins. The symmetry of the difference spectrum, its superb resolution, and unprecedented signal-to-noise ratio in this large protein/receptor complex suggest that this method is generally applicable to study large biopolymeric complexes.

PubMed: 21819146
DOI: 10.1021/ja205480v

実験手法

SOLUTION NMR