2L6Z

haddock model of GATA1NF:Lmo2LIM2-Ldb1LID with FOG

2L6Z の概要

• エントリーDOI: 10.2210/pdb2l6z/pdb
• 関連するPDBエントリー: 2L3K 2L6Y
• 分子名称: Erythroid transcription factor, Zinc finger protein ush, LIM domain only 2, linker, LIM domain-binding protein 1, ... (4 entities in total)
• 機能のキーワード: gata-1, ldb1, lmo2, fog-1, transcription regulation-oncoprotein complex, transcription regulation/oncoprotein
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Nucleus: P17679 Q9VPQ6 P70662
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 19205.38

構造登録者

Wilkinson-White, L.,Gamsjaeger, R.,Dastmalchi, S.,Wienert, B.,Stokes, P.H.,Crossley, M.,Mackay, J.P.,Matthews, J.M. (登録日: 2010-12-01, 公開日: 2011-08-31, 最終更新日: 2024-05-01)

主引用文献

Wilkinson-White, L.,Gamsjaeger, R.,Dastmalchi, S.,Wienert, B.,Stokes, P.H.,Crossley, M.,Mackay, J.P.,Matthews, J.M.
Structural basis of simultaneous recruitment of the transcriptional regulators LMO2 and FOG1/ZFPM1 by the transcription factor GATA1
Proc.Natl.Acad.Sci.USA, 108:14443-14448, 2011

PubMed Abstract: The control of red blood cell and megakaryocyte development by the regulatory protein GATA1 is a paradigm for transcriptional regulation of gene expression in cell lineage differentiation and maturation. Most GATA1-regulated events require GATA1 to bind FOG1, and essentially all GATA1-activated genes are cooccupied by a TAL1/E2A/LMO2/LDB1 complex; however, it is not known whether FOG1 and TAL1/E2A/LMO2/LDB1 are simultaneously recruited by GATA1. Our structural data reveal that the FOG1-binding domain of GATA1, the N finger, can also directly contact LMO2 and show that, despite the small size (< 50 residues) of the GATA1 N finger, both FOG1 and LMO2 can simultaneously bind this domain. LMO2 in turn can simultaneously contact both GATA1 and the DNA-binding protein TAL1/E2A at bipartite E-box/WGATAR sites. Taken together, our data provide the first structural snapshot of multiprotein complex formation at GATA1-dependent genes and support a model in which FOG1 and TAL1/E2A/LMO2/LDB1 can cooccupy E-box/WGATAR sites to facilitate GATA1-mediated activation of gene activation.

PubMed: 21844373
DOI: 10.1073/pnas.1105898108

実験手法

SOLUTION NMR