2L4N

Solution Structure of the Chemokine CCL21

Summary for 2L4N

• Entry DOI: 10.2210/pdb2l4n/pdb
• NMR Information: BMRB: 17245
• Descriptor: C-C motif chemokine 21 (1 entity in total)
• Functional Keywords: slc, 6ckine, exodus-2, ccr7, chemokine, cytokine
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 12519.62

Authors

Veldkamp, C.T.,Peterson, F.C.,Love, M.,Sandberg, J.L. (deposition date: 2010-10-10, release date: 2011-11-02, Last modification date: 2024-11-06)

Primary citation

Love, M.,Sandberg, J.L.,Ziarek, J.J.,Gerarden, K.P.,Rode, R.R.,Jensen, D.R.,McCaslin, D.R.,Peterson, F.C.,Veldkamp, C.T.
Solution structure of CCL21 and identification of a putative CCR7 binding site.
Biochemistry, 51:733-735, 2012

PubMed Abstract: CCL21 is a human chemokine that recruits normal immune cells and metastasizing tumor cells to lymph nodes through activation of the G protein-coupled receptor CCR7. The CCL21 structure solved by NMR contains a conserved chemokine domain followed by an extended, unstructured C-terminus that is not typical of most other chemokines. A sedimentation equilibrium study showed CCL21 to be monomeric. Chemical shift mapping indicates that the CCR7 N-terminus binds to the N-loop and third β-strand of CCL21's chemokine domain. Details of CCL21-receptor recognition may enable structure-based drug discovery of novel antimetastatic agents.

PubMed: 22221265
DOI: 10.1021/bi201601k

Experimental method

SOLUTION NMR