2KXS

ZO1 ZU5 domain in complex with GRINL1A peptide

2KXS の概要

• エントリーDOI: 10.2210/pdb2kxs/pdb
• 関連するPDBエントリー: 2kxr
• 分子名称: Tight junction protein ZO-1,Myocardial zonula adherens protein (1 entity in total)
• 機能のキーワード: beta-barrel, protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15540.85

構造登録者

Wen, W.,Zhang, M. (登録日: 2010-05-12, 公開日: 2011-03-30, 最終更新日: 2024-04-10)

主引用文献

Huo, L.,Wen, W.,Wang, R.,Kam, C.,Xia, J.,Feng, W.,Zhang, M.
Cdc42-dependent formation of the ZO-1/MRCKb complex at the leading edge controls cell migration
Embo J., 30:665-678, 2011

PubMed Abstract: Zonula occludens (ZO)-1 is a multi-domain scaffold protein known to have critical roles in the establishment of cell-cell adhesions and the maintenance of stable tissue structures through the targeting, anchoring, and clustering of transmembrane adhesion molecules and cytoskeletal proteins. Here, we report that ZO-1 directly binds to MRCKβ, a Cdc42 effector kinase that modulates cell protrusion and migration, at the leading edge of migrating cells. Structural studies reveal that the binding of a β hairpin from GRINL1A converts ZO-1 ZU5 into a complete ZU5-fold. A similar interaction mode is likely to occur between ZO-1 ZU5 and MRCKβ. The interaction between ZO-1 and MRCKβ requires the kinase to be primed by Cdc42 due to the closed conformation of the kinase. Formation of the ZO-1/MRCKβ complex enriches the kinase at the lamellae of migrating cells. Disruption of the ZO-1/MRCKβ complex inhibits MRCKβ-mediated cell migration. These results demonstrate that ZO-1, a classical scaffold protein with accepted roles in maintaining cell-cell adhesions in stable tissues, also has an active role in cell migration during processes such as tissue development and remodelling.

PubMed: 21240187
DOI: 10.1038/emboj.2010.353

実験手法

SOLUTION NMR