2KXF

Solution structure of the first two RRM domains of FBP-interacting repressor (FIR)

2KXF の概要

• エントリーDOI: 10.2210/pdb2kxf/pdb
• 関連するPDBエントリー: 2KXH
• 分子名称: Poly(U)-binding-splicing factor PUF60 (1 entity in total)
• 機能のキーワード: rrm, rna binding, protein binding
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21927.02

構造登録者

Cukier, C.D.,Ramos, A.,Hollingworth, D.,Diaz-Moreno, I.,Kelly, G. (登録日: 2010-05-04, 公開日: 2010-08-18, 最終更新日: 2024-05-01)

主引用文献

Cukier, C.D.,Hollingworth, D.,Martin, S.R.,Kelly, G.,Diaz-Moreno, I.,Ramos, A.
Molecular basis of FIR-mediated c-myc transcriptional control.
Nat.Struct.Mol.Biol., 17:1058-1064, 2010

PubMed Abstract: The far upstream element (FUSE) regulatory system promotes a peak in the concentration of c-Myc during cell cycle. First, the FBP transcriptional activator binds to the FUSE DNA element upstream of the c-myc promoter. Then, FBP recruits its specific repressor (FIR), which acts as an on/off transcriptional switch. Here we describe the molecular basis of FIR recruitment, showing that the tandem RNA recognition motifs of FIR provide a platform for independent FUSE DNA and FBP protein binding and explaining the structural basis of the reversibility of the FBP-FIR interaction. We also show that the physical coupling between FBP and FIR is modulated by a flexible linker positioned sequentially to the recruiting element. Our data explain how the FUSE system precisely regulates c-myc transcription and suggest that a small change in FBP-FIR affinity leads to a substantial effect on c-Myc concentration.

PubMed: 20711187
DOI: 10.1038/nsmb.1883

実験手法

SOLUTION NMR