2KUM

Solution structure of the human chemokine CCL27

2KUM の概要

• エントリーDOI: 10.2210/pdb2kum/pdb
• NMR情報: BMRB: 16839
• 分子名称: C-C motif chemokine 27 (1 entity in total)
• 機能のキーワード: ccl27, ctack, chemokine, cytokine, disulfide bond, polymorphism, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: Q9Y4X3
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10165.93

構造登録者

Kirkpatrick, J.P.,Jansma, A.,Hsu, A.,Handel, T.M.,Nietlispach, D. (登録日: 2010-02-22, 公開日: 2010-03-02, 最終更新日: 2024-10-30)

主引用文献

Jansma, A.L.,Kirkpatrick, J.P.,Hsu, A.R.,Handel, T.M.,Nietlispach, D.
NMR analysis of the structure, dynamics, and unique oligomerization properties of the chemokine CCL27.
J.Biol.Chem., 285:14424-14437, 2010

PubMed Abstract: Chemokines have two essential interactions in vivo, with G protein-coupled receptors, which activate intracellular signaling pathways, and with glycosaminoglycans (GAGs), which are involved in cell surface localization and transport. Although it has been shown that chemokines bind and activate their respective G protein-coupled receptors as monomers, many chemokines oligomerize upon GAG binding, and the ability to oligomerize and bind GAGs is required for in vivo function. In this study, we investigated the structure, dynamics, and oligomerization behavior of cutaneous T-cell-attracting chemokine (CTACK, also known as CCL27) by NMR. (15)N relaxation and translational self-diffusion rates indicate that CCL27 oligomerizes, but in contrast to many other chemokines that form relatively discrete oligomers, CCL27 transitions between monomer, dimer, and tetramer species over a relatively narrow concentration range. A three-dimensional structure determination was pursued under conditions where CCL27 is primarily dimeric, revealing the standard motif for a chemokine monomer. Analysis of chemical shift perturbations of (1)H-(15)N HSQC spectra, relaxation-dispersion experiments, and filtered nuclear Overhauser effects suggest that CCL27 does not adopt a discrete CXC or CC dimer motif. Instead, CCL27 has uncommon oligomerization behavior, where several equilibria involving relatively low affinity interactions between different interfaces seem to be simultaneously at work. However, interaction with heparin avidly promotes oligomerization under conditions where CCL27 is monomeric by itself. We hypothesize that the plasticity in the oligomerization state may enable CCL27 to adopt different oligomeric structures, depending on the nature of the GAG binding partner, thereby providing a mechanism for increased diversity and specificity in GAG-binding and GAG-related functions.

PubMed: 20200157
DOI: 10.1074/jbc.M109.091108

実験手法

SOLUTION NMR