2KTO

Spatial structure of Lch-beta peptide from two-component lantibiotic Lichenicidin VK21

2KTO の概要

• エントリーDOI: 10.2210/pdb2kto/pdb
• 関連するPDBエントリー: 1AJ1 1MQX 1MQY 1MQZ 1QOW 1W9N 1WCO 2DDE 2KTN
• 関連するBIRD辞書のPRD_ID: PRD_000198
• 分子名称: LCHB (1 entity in total)
• 機能のキーワード: lichenicidin vk21, lantibiotics, lchb, antibiotic, lanthionine, thioester, two-component lantibiotic system
• 由来する生物種: BACILLUS LICHENIFORMIS
• 細胞内の位置: Secreted (Probable): P86476
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3031.55

構造登録者

Mineev, K.S.,Shenkarev, Z.O.,Ovchinnikova, T.V.,Arseniev, A.S. (登録日: 2010-02-05, 公開日: 2010-07-21, 最終更新日: 2024-07-10)

主引用文献

Shenkarev, Z.O.,Finkina, E.I.,Nurmukhamedova, E.K.,Balandin, S.V.,Mineev, K.S.,Nadezhdin, K.D.,Yakimenko, Z.A.,Tagaev, A.A.,Temirov, Y.V.,Arseniev, A.S.,Ovchinnikova, T.V.
Isolation, Structure Elucidation, and Synergistic Antibacterial Activity of a Novel Two-Component Lantibiotic Lichenicidin from Bacillus Licheniformis Vk21.
Biochemistry, 49:6462-, 2010

PubMed Abstract: A novel synergetic lantibiotic pair, Lchalpha (3249.51 Da) and Lchbeta (3019.36 Da), termed lichenicidin VK21, was isolated from the producer strain Bacillus licheniformis VK21. Chemical and spatial structures of Lchalpha and Lchbeta were determined. Each peptide contains 31 amino acid residues linked by 4 intramolecular thioether bridges and the N-terminal 2-oxobutyryl group. Spatial structures of Lchalpha and Lchbeta were studied by NMR spectroscopy in methanol solution. The Lchalpha peptide displays structural homology with mersacidin-like lantibiotics and involves relatively well-structured N- and C-terminal domains connected by a flexible loop stabilized by a thioether bridge Ala11-S-Ala21. In contrast, the Lchbeta peptide represents a prolonged hydrophobic alpha-helix flanked with more flexible N- and C-terminal domains. A lantibiotic cluster of the Bacillus licheniformis VK21 genome which comprises the structural genes, lchA1 and lchA2, encoding the lantibiotics precursors, as well as the gene of a modifying enzyme lchM1, was amplified and sequenced. The mature peptides, Lchalpha and Lchbeta, interact synergistically to possess antibiotic activity against Gram-positive bacteria within a nanomolar concentration range, though the individual peptides were shown to be active at micromolar concentrations. Our results afford molecular insight into the mechanism of lichenicidin VK21 action.

PubMed: 20578714
DOI: 10.1021/BI100871B

実験手法

SOLUTION NMR