2KQZ

Solution structure of the Rpn13 DEUBAD domain

2KQZ の概要

• エントリーDOI: 10.2210/pdb2kqz/pdb
• 関連するPDBエントリー: 2R2Y 2Z4D 2Z59 3IHR
• 分子名称: Proteasomal ubiquitin receptor ADRM1 (1 entity in total)
• 機能のキーワード: proteasome, uch37-binding domain, protein binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: Q16186
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16276.11

構造登録者

Chen, X.,Lee, B.,Finley, D.,Walters, K.J. (登録日: 2009-11-25, 公開日: 2010-05-26, 最終更新日: 2024-05-01)

主引用文献

Chen, X.,Lee, B.H.,Finley, D.,Walters, K.J.
Structure of Proteasome Ubiquitin Receptor hRpn13 and Its Activation by the Scaffolding Protein hRpn2.
Mol.Cell, 38:404-415, 2010

PubMed Abstract: Rpn13 is a subunit of the proteasome that serves as a receptor for both ubiquitin and Uch37, one of the proteasome's three deubiquitinating enzymes. We have determined the structure of full-length human Rpn13 (hRpn13). Unexpectedly, Rpn13's ubiquitin- and Uch37-binding domains pack against each other when it is not incorporated into the proteasome. This intramolecular interaction reduces hRpn13's affinity for ubiquitin. We find that hRpn13 binding to the proteasome scaffolding protein hRpn2/S1 abrogates its interdomain interactions, thus activating hRpn13 for ubiquitin binding. hRpn13's Uch37-binding domain, a previously unknown fold, contains nine alpha helices. We have mapped its Uch37-binding surface to a region rich in charged amino acids. Altogether, our results provide mechanistic insights into hRpn13's functional activities with Uch37 and ubiquitin and suggest that its role as a ubiquitin receptor is finely tuned for proteasome targeting.

PubMed: 20471946
DOI: 10.1016/j.molcel.2010.04.019

実験手法

SOLUTION NMR