2KQB

First PBZ domain of human APLF protein

Summary for 2KQB

• Entry DOI: 10.2210/pdb2kqb/pdb
• Related: 2KQC 2KQD 2KQE
• NMR Information: BMRB: 16596
• Descriptor: Aprataxin and PNK-like factor, ZINC ION (2 entities in total)
• Functional Keywords: adp-ribosylation, dna damage, dna repair, metal-binding, nucleotide-binding, nucleus, zinc, zinc-finger, lyase
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 10118.47

Authors

Neuhaus, D.,Eustermann, S.,Brockmann, C.,Yang, J. (deposition date: 2009-11-04, release date: 2010-01-19, Last modification date: 2024-05-08)

Primary citation

Eustermann, S.,Brockmann, C.,Mehrotra, P.V.,Yang, J.C.,Loakes, D.,West, S.C.,Ahel, I.,Neuhaus, D.
Solution structures of the two PBZ domains from human APLF and their interaction with poly(ADP-ribose).
Nat.Struct.Mol.Biol., 17:241-243, 2010

PubMed Abstract: Addition of poly(ADP-ribose) (PAR) is an important post-translational modification in higher eukaryotes. Several DNA repair and checkpoint proteins possess specific PAR-binding zinc-finger (PBZ) modules critical for function. Here, we present solution structures of the two PBZ modules of aprataxin and PNK-like factor (APLF), revealing a novel type of zinc finger. By combining in vivo PAR-binding data with NMR interaction data using PAR fragments, we propose a structural basis for PBZ-PAR recognition.

PubMed: 20098424
DOI: 10.1038/nsmb.1747

Experimental method

SOLUTION NMR