2KOG

lipid-bound synaptobrevin solution NMR structure

2KOG の概要

• エントリーDOI: 10.2210/pdb2kog/pdb
• 分子名称: Vesicle-associated membrane protein 2 (1 entity in total)
• 機能のキーワード: synaptobrevin, vamp2, dpc micelle, snare, coiled coil, membrane fusion, transmembrane, synaptic vesicle, membrane protein
• 由来する生物種: Rattus norvegicus (rat)
• 細胞内の位置: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein: P63045
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12986.01

構造登録者

Ellena, J.F.,Liang, B.,Wiktor, M.,Stein, A.,Cafiso, D.S.,Jahn, R.,Tamm, L.K. (登録日: 2009-09-22, 公開日: 2009-12-01, 最終更新日: 2024-05-22)

主引用文献

Ellena, J.F.,Liang, B.,Wiktor, M.,Stein, A.,Cafiso, D.S.,Jahn, R.,Tamm, L.K.
Dynamic structure of lipid-bound synaptobrevin suggests a nucleation-propagation mechanism for trans-SNARE complex formation.
Proc.Natl.Acad.Sci.USA, 106:20306-20311, 2009

PubMed Abstract: The synaptic vesicle protein synaptobrevin engages with syntaxin and SNAP-25 to form the SNARE complex, which drives membrane fusion in neuronal exocytosis. In the SNARE complex, the SNARE motif of synaptobrevin forms a 55-residue helix, but it has been assumed to be mostly unstructured in its prefusion form. NMR data for full-length synaptobrevin in dodecylphosphocholine micelles reveals two transient helical segments flanked by natively disordered regions and a third more stable helix. Transient helix I comprises the most N-terminal part of the SNARE motif, transient helix II extends the SNARE motif into the juxtamembrane region, and the more stable helix III is the transmembrane domain. These helices may have important consequences for SNARE complex folding and fusion: helix I likely forms a nucleation site, the C-terminal disordered SNARE motif may act as a folding arrest signal, and helix II likely couples SNARE complex folding and fusion.

PubMed: 19918058
DOI: 10.1073/pnas.0908317106

実験手法

SOLUTION NMR