2KNA

Solution structure of UBA domain of XIAP

2KNA の概要

• エントリーDOI: 10.2210/pdb2kna/pdb
• NMR情報: BMRB: 16478
• 分子名称: Baculoviral IAP repeat-containing protein 4 (1 entity in total)
• 機能のキーワード: xiap, uba, apoptosis, ligase, metal-binding, phosphoprotein, protease inhibitor, thiol protease inhibitor, ubl conjugation pathway, zinc-finger
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P98170
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11873.37

構造登録者

Hui, S.K.,Tse, M.K.,Sze, K.H. (登録日: 2009-08-20, 公開日: 2010-09-01, 最終更新日: 2024-05-01)

主引用文献

Hui, S.K.,Tse, M.K.,Yang, Y.,Wong, B.C.,Sze, K.H.
Backbone and side-chain 1H, 13C and 15N assignments of the ubiquitin-associated domain of human X-linked inhibitor of apoptosis protein
Biomol.Nmr Assign., 4:13-15, 2010

PubMed Abstract: X-linked inhibitor of apoptosis protein (XIAP), a leading member of the family of inhibitor of apoptosis (IAP) proteins, is considered as the most potent and versatile inhibitor of caspases and apoptosis. It has been reported that XIAP is frequently overexpressed in cancer and its expression level is implicated in contributing to tumorigenesis, disease progression, chemoresistance and poor patient-survival. Therefore, XIAP is one of the leading targets in drug development for cancer therapy. Recently, based on bioinformatics study, a previously unrecognized but evolutionarily conserved ubiquitin-associated (UBA) domain in IAPs was identified. The UBA domain is found to be essential for the oncogenic potential of IAP, to maintain endothelial cell survival and to protect cells from TNF-alpha-induced apoptosis. Moreover, the UBA domain is required for XIAP to activate NF-kappaB. In the present study, we report the near complete resonance assignments of the UBA domain-containing region of human XIAP protein. Secondary structure prediction based on chemical shift index (CSI) analysis reveals that the protein is predominately alpha-helical, which is consistent with the structures of known UBA proteins.

PubMed: 19916060
DOI: 10.1007/s12104-009-9197-x

実験手法

SOLUTION NMR