2KMJ

High resolution NMR solution structure of a complex of HIV-2 TAR RNA and a synthetic tripeptide in a 1:2 stoichiometry

2KMJ の概要

• エントリーDOI: 10.2210/pdb2kmj/pdb
• NMR情報: BMRB: 16431
• 分子名称: RNA (28-MER), Pyrimidinylpeptide (2 entities in total)
• 機能のキーワード: rna-ligand complex, binding stoichiometry, hiv-2 tar, rna-peptide complex, rna/peptide
• 由来する生物種: Human immunodeficiency virus 2; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 9955.52

構造登録者

Ferner, J.,Suhartono, M.,Breitung, S.,Jonker, H.R.A.,Hennig, M.,Woehnert, J.,Goebel, M.,Schwalbe, H. (登録日: 2009-07-30, 公開日: 2009-08-18, 最終更新日: 2024-11-27)

主引用文献

Ferner, J.,Suhartono, M.,Breitung, S.,Jonker, H.R.A.,Hennig, M.,Wohnert, J.,Gobel, M.,Schwalbe, H.
Structures of HIV TAR RNA-ligand complexes reveal higher binding stoichiometries.
Chembiochem, 10:1490-1494, 2009

PubMed Abstract: Target TAR by NMR: Tripeptides containing arginines as terminal residues and non-natural amino acids as central residues are good leads for drug design to target the HIV trans-activation response element (TAR). The structural characterization of the RNA-ligand complex by NMR spectroscopy reveals two specific binding sites that are located at bulge residue U23 and around the pyrimidine-stretch U40-C41-U42 directly adjacent to the bulge.

PubMed: 19444830
DOI: 10.1002/cbic.200900220

実験手法

SOLUTION NMR