2KLH

NMR Structure of RCL in complex with GMP

2KLH の概要

• エントリーDOI: 10.2210/pdb2klh/pdb
• 分子名称: c-Myc-responsive protein Rcl, GUANOSINE-5'-MONOPHOSPHATE (2 entities in total)
• 機能のキーワード: protein, gmp, n-glycosidase, nucleus, phosphoprotein, hydrolase
• 由来する生物種: Rattus norvegicus (rat)
• 細胞内の位置: Cytoplasm (By similarity): O35820
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34987.06

構造登録者

Padilla, A.,Yang, Y.,Labesse, G.,Zhang, C.,Kaminski, P.A. (登録日: 2009-07-02, 公開日: 2009-10-20, 最終更新日: 2024-05-29)

主引用文献

Yang, Y.,Padilla, A.,Zhang, C.,Labesse, G.,Kaminski, P.A.
Structural characterization of the mammalian deoxynucleotide N-hydrolase Rcl and its stabilizing interactions with two inhibitors
J.Mol.Biol., 394:435-447, 2009

PubMed Abstract: The gene Rcl encodes a deoxynucleoside 5'-monophosphate N-glycosidase that catalyzes the hydrolysis of the N-glycosidic bond of the nucleotide to give deoxyribose 5-phosphate and a nucleobase, preferentially a purine. This enzyme is over-expressed in several cancers, and its rate of expression is correlated to the degree of aggressiveness of tumors, which makes it a new and attractive therapeutic target. We describe here its structural characterization in the presence of two inhibitory substrate mimics. One of these ligands corresponds to the monophosphorylated form of acyclovir, which is used in the clinic. This study reveals an important ligand-induced stabilization of the dimer structure of the enzyme. The original structural features of Rcl will be helpful for designing new inhibitors.

PubMed: 19822152
DOI: 10.1016/j.jmb.2009.10.004

実験手法

SOLUTION NMR