2KEI

Refined Solution Structure of a Dimer of LAC repressor DNA-Binding domain complexed to its natural operator O1

Summary for 2KEI

• Entry DOI: 10.2210/pdb2kei/pdb
• Related: 1L1M 2KEJ 2KEK
• Descriptor: Lactose operon repressor, DNA (5'-D(*GP*AP*AP*TP*TP*GP*TP*GP*AP*GP*CP*GP*GP*AP*TP*AP*AP*CP*AP*AP*TP*TP*T)-3'), DNA (5'-D(P*AP*AP*AP*TP*TP*GP*TP*TP*AP*TP*CP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*TP*C)-3') (3 entities in total)
• Functional Keywords: lac repressor, lac operators, protein-dna complex, dna-binding, repressor, transcription, transcription regulation, transcription-dna complex, transcription/dna
• Biological source: Escherichia coli
• Total number of polymer chains: 4
• Total formula weight: 27763.69

Authors

Romanuka, J.,Folkers, G.,Biris, N.,Tishchenko, E.,Wienk, H.,Kaptein, R.,Boelens, R. (deposition date: 2009-01-30, release date: 2009-05-19, Last modification date: 2021-10-20)

• Primary citation: Romanuka, J.,Folkers, G.E.,Biris, N.,Tishchenko, E.,Wienk, H.,Bonvin, A.M.,Kaptein, R.,Boelens, R.; Specificity and affinity of Lac repressor for the auxiliary operators O2 and O3 are explained by the structures of their protein-DNA complexes.; J.Mol.Biol., 390:478-489, 2009; PubMed: 19450607; DOI: 10.1016/j.jmb.2009.05.022

Experimental method

SOLUTION NMR