2KE8

NMR solution structure of metal-modified DNA

2KE8 の概要

• エントリーDOI: 10.2210/pdb2ke8/pdb
• 関連するPDBエントリー: 2K67 2K68 2K69
• 分子名称: DNA (5'-D(*TP*TP*AP*AP*TP*TP*TP*(D33)P*(D33)P*(D33)P*AP*AP*AP*TP*TP*AP*A)-3'), SILVER ION (2 entities in total)
• 機能のキーワード: dna, artificial nucleobase, imidazole nucleoside, ag+
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 10354.23

構造登録者

Johannsen, S.,Duepre, N.,Boehme, D.,Mueller, J.,Sigel, R.K.O. (登録日: 2009-01-27, 公開日: 2010-02-09, 最終更新日: 2024-05-01)

主引用文献

Johannsen, S.,Megger, N.,Bohme, D.,Sigel, R.K.,Muller, J.
Solution structure of a DNA double helix with consecutive metal-mediated base pairs.
Nat.Chem., 2:229-234, 2010

PubMed Abstract: Metal-mediated base pairs represent a powerful tool for the site-specific functionalization of nucleic acids with metal ions. The development of applications of the metal-modified nucleic acids will depend on the availability of structural information on these double helices. We present here the NMR solution structure of a self-complementary DNA oligonucleotide with three consecutive imidazole nucleotides in its centre. In the absence of transition-metal ions, a hairpin structure is adopted with the artificial nucleotides forming the loop. In the presence of Ag(i) ions, a duplex comprising three imidazole-Ag(+)-imidazole base pairs is formed. Direct proof for the formation of metal-mediated base pairs was obtained from ¹J(¹⁵N,¹⁰⁷/¹⁰⁹Ag) couplings upon incorporation of ¹⁵N-labelled imidazole. The duplex adopts a B-type conformation with only minor deviations in the region of the artificial bases. This work represents the first structural characterization of a metal-modified nucleic acid with a continuous stretch of metal-mediated base pairs.

PubMed: 21124482
DOI: 10.1038/nchem.512

実験手法

SOLUTION NMR