2KDT

PC1/3 DCSG sorting domain structure in DPC

Summary for 2KDT

• Entry DOI: 10.2210/pdb2kdt/pdb
• Descriptor: Neuroendocrine convertase 1 (1 entity in total)
• Functional Keywords: secretory granules, prohorme convertase, calcium, cleavage on pair of basic residues, cytoplasmic vesicle, glycoprotein, hydrolase, protease, serine protease, zymogen, protein transport
• Biological source: Mus musculus (mouse)
• Cellular location: Cytoplasmic vesicle, secretory vesicle: P63239
• Total number of polymer chains: 1
• Total formula weight: 5275.73

Authors

Dikeakos, J.D.,Di Lello, P.,Lacombe, M.J.,Ghirlando, R.,Legault, P.,Reudelhuber, T.L.,Omichinski, J.G. (deposition date: 2009-01-19, release date: 2009-04-07, Last modification date: 2024-05-22)

Primary citation

Dikeakos, J.D.,Di Lello, P.,Lacombe, M.J.,Ghirlando, R.,Legault, P.,Reudelhuber, T.L.,Omichinski, J.G.
Functional and structural characterization of a dense core secretory granule sorting domain from the PC1/3 protease.
Proc.Natl.Acad.Sci.USA, 106:7408-7413, 2009

PubMed Abstract: Several peptide hormones are initially synthesized as inactive precursors. It is only on entry of these prohormones and their processing proteases into dense core secretory granules (DCSGs) that the precursors are cleaved to generate their active forms. Prohormone convertase (PC)1/3 is a processing protease that is targeted to DCSGs. The signal for targeting PC1/3 to DCSGs resides in its carboxy-terminal tail (PC1/3(617-753)), where 3 regions (PC1/3(617-625), PC1/3(665-682), and PC1/3(711-753)) are known to aid in sorting and membrane association. In this article, we have determined a high-resolution structure of the extreme carboxy-terminal sorting domain, PC1/3(711-753) in micelles by NMR spectroscopy. PC1/3(711-753) contains 2 alpha helices located between residues 722-728 and 738-750. Functional assays demonstrate that the second helix (PC1/3(738-750)) is necessary and sufficient to target a constitutively secreted protein to granules, and that L(745) anchors a hydrophobic patch that is critical for sorting. Also, we demonstrate that calcium binding by the second helix of PC1/3(711-753) promotes aggregation of the domain via the hydrophobic patch centered on L(745). These results provide a structure-function analysis of a DCSG-sorting domain, and reveal the importance of a hydrophobic patch and calcium binding in controlling the sorting of proteins containing alpha helices to DCSGs.

PubMed: 19376969
DOI: 10.1073/pnas.0809576106

Experimental method

SOLUTION NMR