2KBT

Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method

Summary for 2KBT

• Entry DOI: 10.2210/pdb2kbt/pdb
• NMR Information: BMRB: 16053
• Descriptor: Proto-oncogene vav,Immunoglobulin G-binding protein G (1 entity in total)
• Functional Keywords: sortase, protein ligation, gb1, intein, inset, solubility enhancement, signaling protein
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 1
• Total formula weight: 15974.47

Authors

Kumeta, H.,Kobashigawa, Y.,Ogura, K.,Inagaki, F. (deposition date: 2008-12-07, release date: 2009-02-03, Last modification date: 2024-05-15)

Primary citation

Kobashigawa, Y.,Kumeta, H.,Ogura, K.,Inagaki, F.
Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method
J.Biomol.Nmr, 43:145-150, 2009

PubMed Abstract: Sample solubility is essential for structural studies of proteins by solution NMR. Attachment of a solubility enhancement tag, such as GB1, MBP and thioredoxin, to a target protein has been used for this purpose. However, signal overlap of the tag with the target protein often made the spectral analysis difficult. Here we report a sortase-mediated protein ligation method to eliminate NMR signals arising from the tag by preparing the isotopically labeled target protein attached with the non-labeled GB1 tag at the C-terminus.

PubMed: 19140010
DOI: 10.1007/s10858-008-9296-5

Experimental method

SOLUTION NMR