2KBT

Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method

2KBT の概要

• エントリーDOI: 10.2210/pdb2kbt/pdb
• NMR情報: BMRB: 16053
• 分子名称: Proto-oncogene vav,Immunoglobulin G-binding protein G (1 entity in total)
• 機能のキーワード: sortase, protein ligation, gb1, intein, inset, solubility enhancement, signaling protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15974.47

構造登録者

Kumeta, H.,Kobashigawa, Y.,Ogura, K.,Inagaki, F. (登録日: 2008-12-07, 公開日: 2009-02-03, 最終更新日: 2024-05-15)

主引用文献

Kobashigawa, Y.,Kumeta, H.,Ogura, K.,Inagaki, F.
Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method
J.Biomol.Nmr, 43:145-150, 2009

PubMed Abstract: Sample solubility is essential for structural studies of proteins by solution NMR. Attachment of a solubility enhancement tag, such as GB1, MBP and thioredoxin, to a target protein has been used for this purpose. However, signal overlap of the tag with the target protein often made the spectral analysis difficult. Here we report a sortase-mediated protein ligation method to eliminate NMR signals arising from the tag by preparing the isotopically labeled target protein attached with the non-labeled GB1 tag at the C-terminus.

PubMed: 19140010
DOI: 10.1007/s10858-008-9296-5

実験手法

SOLUTION NMR