2K9O
Solution structure of Vm24 synthetic scorpion toxin
Summary for 2K9O
| Entry DOI | 10.2210/pdb2k9o/pdb |
| NMR Information | BMRB: 15992 |
| Descriptor | Vm24 SCORPION toxin (1 entity in total) |
| Functional Keywords | alfa/beta scaffold, beta sheet, alfa helix, scorpion k+ toxin, vm24, toxin |
| Total number of polymer chains | 1 |
| Total formula weight | 3879.69 |
| Authors | del Rio-Portilla, F.,Hernandez-Lopez, R.,Possani-Postay, L.,Gurrola, G. (deposition date: 2008-10-20, release date: 2009-11-03, Last modification date: 2024-11-27) |
| Primary citation | Gurrola, G.B.,Hernandez-Lopez, R.A.,Rodriguez de la Vega, R.C.,Varga, Z.,Batista, C.V.,Salas-Castillo, S.P.,Panyi, G.,del Rio-Portilla, F.,Possani, L.D. Structure, function, and chemical synthesis of Vaejovis mexicanus peptide 24: a novel potent blocker of Kv1.3 potassium channels of human T lymphocytes. Biochemistry, 51:4049-4061, 2012 Cited by PubMed Abstract: Animal venoms are rich sources of ligands for studying ion channels and other pharmacological targets. Proteomic analyses of the soluble venom from the Mexican scorpion Vaejovis mexicanus smithi showed that it contains more than 200 different components. Among them, a 36-residue peptide with a molecular mass of 3864 Da (named Vm24) was shown to be a potent blocker of Kv1.3 of human lymphocytes (K(d) ∼ 3 pM). The three-dimensional solution structure of Vm24 was determined by nuclear magnetic resonance, showing the peptide folds into a distorted cystine-stabilized α/β motif consisting of a single-turn α-helix and a three-stranded antiparallel β-sheet, stabilized by four disulfide bridges. The disulfide pairs are formed between Cys6 and Cys26, Cys12 and Cys31, Cys16 and Cys33, and Cys21 and Cys36. Sequence analyses identified Vm24 as the first example of a new subfamily of α-type K(+) channel blockers (systematic number α-KTx 23.1). Comparison with other Kv1.3 blockers isolated from scorpions suggests a number of structural features that could explain the remarkable affinity and specificity of Vm24 toward Kv1.3 channels of lymphocytes. PubMed: 22540187DOI: 10.1021/bi300060n PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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