2JX2

Solution conformation of RNA-bound NELF-E RRM

2JX2 の概要

• エントリーDOI: 10.2210/pdb2jx2/pdb
• NMR情報: BMRB: 15543
• 分子名称: Negative elongation factor E (1 entity in total)
• 機能のキーワード: nelfe-rrm, coiled coil, nucleus, phosphorylation, repressor, rna-binding, transcription, transcription regulation, ubl conjugation
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13384.09

構造登録者

Jampani, N.,Schweimer, K.,Wenzel, S.,Woehrl, B.M.,Roesch, P. (登録日: 2007-11-02, 公開日: 2008-04-08, 最終更新日: 2024-05-08)

主引用文献

Rao, J.N.,Schweimer, K.,Wenzel, S.,Wohrl, B.M.,Rosch, P.
NELF-E RRM Undergoes Major Structural Changes in Flexible Protein Regions on Target RNA Binding
Biochemistry, 47:3756-3761, 2008

PubMed Abstract: The E subunit of the human heterotetrameric negative transcription elongation factor (NELF-E) contains a canonical betaalphabetabetaalphabeta RNA recognition motif (RRM) that binds to a wide variety of RNA sequences. These induce very similar conformational changes in the RRM as determined by nuclear magnetic resonance spectroscopy. Although the RNA binding interface of a canonical RRM is mainly located at its beta-sheet surface, for NELF-E RRM large chemical shift perturbations are observed for residues in the flexible C-terminal region and the loop between beta 3 and alpha 2, and both regions are distant from the interface. We determined the solution structure of single-stranded transactivator responsive element (TAR) RNA-bound NELF-E RRM. This structure clearly shows that RNA binding to NELF-E RRM induces formation of a helix in the C-terminus. The RNA-bound form of NELF-E RRM is very similar to the RNA-bound form of U1A RRM, although the C-terminus of the NELF-E RRM is unstructured in the free protein, whereas it is helical in the U1A protein. Thus, RNA binding to NELF-E RRM induces a conformational change toward the U1A structure, resulting in highly similar RNA binding conformations for both proteins.

PubMed: 18303858
DOI: 10.1021/bi702429m

実験手法

SOLUTION NMR