2JNB

Solution Structure of RNA-binding protein 15.5K

2JNB の概要

• エントリーDOI: 10.2210/pdb2jnb/pdb
• 分子名称: NHP2-like protein 1 (1 entity in total)
• 機能のキーワード: splicing, kink-turn rna-binding protein, nhpx, rna binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus, nucleolus: P55769
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16025.50

構造登録者

Soss, S.E.,Flynn, P.F. (登録日: 2007-01-04, 公開日: 2007-12-18, 最終更新日: 2023-12-20)

主引用文献

Soss, S.E.,Flynn, P.F.
Functional Implications for a Prototypical K-Turn Binding Protein from Structural and Dynamical Studies of 15.5K
Biochemistry, 46:14979-14986, 2007

PubMed Abstract: The kink-turn (K-turn) motif is recognized and bound by a family of proteins that act as nucleation factors for ribonucleoparticle assembly. The binding of various proteins to a conserved RNA structural motif known as the K-turn has been shown to be an important component of regulation in the ribosome, in the spliceosome, and in RNA modification. 15.5K is a prototypical example of a K-turn binding protein, which has been shown to bind the 5'-U4 stem-loop of the spliceosome and the box C/D motif. We describe the solution NMR structure of free 15.5K, as well as studies of conformational flexibility from 15N NMR relaxation and H/D exchange experiments. The protein appears well-structured aside from conformational fluctuation in alpha3. Flexibility in fast time scale motions and the observation of limited intermediate and slow motions further characterize the free protein and may suggest local contributions to recognition and binding.

PubMed: 18044964
DOI: 10.1021/bi701254q

実験手法

SOLUTION NMR