2JJ2

The Structure of F1-ATPase inhibited by quercetin.

2JJ2 の概要

• エントリーDOI: 10.2210/pdb2jj2/pdb
• 関連するPDBエントリー: 1BMF 1COW 1E1Q 1E1R 1E79 1EFR 1H8E 1H8H 1NBM 1OHH 1QO1 1W0J 1W0K 2CK3 2JDI 2JIZ 2JJ1 2UYS
• 分子名称: ATP SYNTHASE SUBUNIT ALPHA HEART ISOFORM, 3,5,7,3',4'-PENTAHYDROXYFLAVONE, ATP SYNTHASE SUBUNIT BETA, ... (11 entities in total)
• 機能のキーワード: atp phosphorylase, hydrolase, atp synthesis
• 由来する生物種: BOS TAURUS (BOVINE); 詳細
• 細胞内の位置: Mitochondrion inner membrane (By similarity): P19483; Mitochondrion: P00829 P05631
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 710042.28

構造登録者

Gledhill, J.R.,Montgomery, M.G.,Leslie, A.G.W.,Walker, J.E. (登録日: 2007-07-03, 公開日: 2007-08-21, 最終更新日: 2023-12-13)

主引用文献

Gledhill, J.R.,Montgomery, M.G.,Leslie, A.G.W.,Walker, J.E.
Mechanism of Inhibition of Bovine F1-ATPase by Resveratrol and Related Polyphenols.
Proc.Natl.Acad.Sci.USA, 104:13632-, 2007

PubMed Abstract: The structures of F(1)-ATPase from bovine heart mitochondria inhibited with the dietary phytopolyphenol, resveratrol, and with the related polyphenols quercetin and piceatannol have been determined at 2.3-, 2.4- and 2.7-A resolution, respectively. The inhibitors bind to a common site in the inside surface of an annulus made from loops in the three alpha- and three beta-subunits beneath the "crown" of beta-strands in their N-terminal domains. This region of F(1)-ATPase forms a bearing to allow the rotation of the tip of the gamma-subunit inside the annulus during catalysis. The binding site is a hydrophobic pocket between the C-terminal tip of the gamma-subunit and the beta(TP) subunit, and the inhibitors are bound via H-bonds mostly to their hydroxyl moieties mediated by bound water molecules and by hydrophobic interactions. There are no equivalent sites between the gamma-subunit and either the beta(DP) or the beta(E) subunit. The inhibitors probably prevent both the synthetic and hydrolytic activities of the enzyme by blocking both senses of rotation of the gamma-subunit. The beneficial effects of dietary resveratrol may derive in part by preventing mitochondrial ATP synthesis in tumor cells, thereby inducing apoptosis.

PubMed: 17698806
DOI: 10.1073/PNAS.0706290104

実験手法

X-RAY DIFFRACTION (2.4 Å)