2JHN
3-methyladenine dna-glycosylase from Archaeoglobus fulgidus
Summary for 2JHN
| Entry DOI | 10.2210/pdb2jhn/pdb |
| Related | 2JHJ |
| Descriptor | 3-METHYLADENINE DNA-GLYCOSYLASE, MERCURIBENZOIC ACID, MERCURY (II) ION, ... (7 entities in total) |
| Functional Keywords | alka, dna repair, n1-methyladenine, n3-methylcytosine, hyperthermophiles, archaeoglobus fulgidus, hydrolase |
| Biological source | ARCHAEOGLOBUS FULGIDUS |
| Total number of polymer chains | 2 |
| Total formula weight | 70848.91 |
| Authors | Leiros, I.,Nabong, M.P.,Grosvik, K.,Ringvoll, J.,Haugland, G.T.,Uldal, L.,Reite, K.,Olsbu, I.K.,Knaevelsrud, I.,Moe, E.,Andersen, O.A.,Birkeland, N.K.,Ruoff, P.,Klungland, A.,Bjelland, S. (deposition date: 2007-02-22, release date: 2007-04-10, Last modification date: 2024-05-08) |
| Primary citation | Leiros, I.,Nabong, M.P.,Grosvik, K.,Ringvoll, J.,Haugland, G.T.,Uldal, L.,Reite, K.,Olsbu, I.K.,Knaevelsrud, I.,Moe, E.,Andersen, O.A.,Birkeland, N.K.,Ruoff, P.,Klungland, A.,Bjelland, S. Structural Basis for Enzymatic Excision of N1-Methyladenine and N3-Methylcytosine from DNA Embo J., 26:2206-, 2007 Cited by PubMed Abstract: N(1)-methyladenine (m(1)A) and N(3)-methylcytosine (m(3)C) are major toxic and mutagenic lesions induced by alkylation in single-stranded DNA. In bacteria and mammals, m(1)A and m(3)C were recently shown to be repaired by AlkB-mediated oxidative demethylation, a direct DNA damage reversal mechanism. No AlkB gene homologues have been identified in Archaea. We report that m(1)A and m(3)C are repaired by the AfAlkA base excision repair glycosylase of Archaeoglobus fulgidus, suggesting a different repair mechanism for these lesions in the third domain of life. In addition, AfAlkA was found to effect a robust excision of 1,N(6)-ethenoadenine. We present a high-resolution crystal structure of AfAlkA, which, together with the characterization of several site-directed mutants, forms a molecular rationalization for the newly discovered base excision activity. PubMed: 17396151DOI: 10.1038/SJ.EMBOJ.7601662 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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