2JGU

crystal structure of DNA-directed DNA polymerase

2JGU の概要

• エントリーDOI: 10.2210/pdb2jgu/pdb
• 分子名称: DNA POLYMERASE, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: dna-directed dna polymerase, pfu, nuclease, hydrolase, transferase, dna-binding, endonuclease, dna replication, nucleotidyltransferase
• 由来する生物種: PYROCOCCUS FURIOSUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 90370.13

構造登録者

Kim, D.U.,Cho, H.S. (登録日: 2007-02-15, 公開日: 2008-04-22, 最終更新日: 2024-10-09)

主引用文献

Kim, S.W.,Kim, D.U.,Kim, J.K.,Kang, L.W.,Cho, H.S.
Crystal Structure of Pfu, the High Fidelity DNA Polymerase from Pyrococcus Furiosus.
Int.J.Biol.Macromol., 42:356-, 2008

PubMed Abstract: We have determined a 2.6A resolution crystal structure of Pfu DNA polymerase, the most commonly used high fidelity PCR enzyme, from Pyrococcus furiosus. Although the structures of Pfu and KOD1 are highly similar, the structure of Pfu elucidates the electron density of the interface between the exonuclease and thumb domains, which has not been previously observed in the KOD1 structure. The interaction of these two domains is known to coordinate the proofreading and polymerization activity of DNA polymerases, especially via H147 that is present within the loop (residues 144-158) of the exonuclease domain. In our structure of Pfu, however, E148 rather than H147 is located at better position to interact with the thumb domain. In addition, the structural analysis of Pfu and KOD1 shows that both the Y-GG/A and beta-hairpin motifs of Pfu are found to differ with that of KOD1, and may explain differences in processivity. This information enables us to better understand the mechanisms of polymerization and proofreading of DNA polymerases.

PubMed: 18355915
DOI: 10.1016/J.IJBIOMAC.2008.01.010

実験手法

X-RAY DIFFRACTION (2.6 Å)